Defining the functions of trans-SNARE pairs

被引：287

作者：

Ungermann, C ^{[1
]}

Sato, K ^{[1
]}

Wickner, W ^{[1
]}

机构：

[1] Dartmouth Coll, Sch Med, Dept Biochem, Hanover, NH 03755 USA

来源：

NATURE | 1998年 / 396卷 / 6711期

关键词：

D O I：

10.1038/25069

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The homotypic fusion of yeast vacuoles includes a 'docking' step, which we show here to consist of two sequential reactions: a reversible 'tethering' mediated by the GTPase Ypt7, and 'SNARE pairing', in which SNARE proteins from opposite membranes form a complex in trans. The function of this trans-SNARE complex must be transient, as the complex can be disassembled by excess Sec18 in the presence of Sec17 and ATP without influencing the fusion rate. These data Indicate that SNARE pairing may transiently signal to downstream factors, leading to fusion.

引用

页码：543 / 548

页数：6

共 34 条

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