Localisation of glutathione and homoglutathione in Medicago truncatula is correlated to a differential expression of genes involved in their synthesis

被引:40
作者
Frendo, P [1 ]
Gallesi, D [1 ]
Turnbull, R [1 ]
Van de Sype, G [1 ]
Hérouart, D [1 ]
Puppo, A [1 ]
机构
[1] Univ Nice, CNRS, Lab Biol Vegetale & Microbiol, ERS 590, F-06108 Nice 2, France
关键词
D O I
10.1046/j.1365-313X.1999.00367.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Glutathione (GSH) and homoglutathione (hGSH) were quantified in Medicago truncatula during plant development. hGSH was detectable only 48 h after seed germination whereas GSH was present in the dry seeds, indicating that only GSH is used for sulphur storage in seeds. The hGSH was detectable only in the underground part of mature plants whereas GSH was present in all the organs. gamma-EC synthetase (gamma-ECS) and GSH synthetase (GSHS) activities were found in roots and leaves whereas hGSH synthetase (hGSHS) was found only in roots. Full-length cDNA encoding gamma-ECS and two partial cDNAs (gshs1 and gshs2) showing high identity with GSHS were isolated in M. truncatula. High gamma-ECS activity was detected in protein extracts of a gamma-ECS-deficient E. coli strain expressing the M. truncatula gamma-ECS. Northern blot analysis showed that the gamma-ECS gene was similarly expressed in all the mature plant organs tested, whereas gshs1 had a higher expression in leaves and flowers and gshs2 was preferentially expressed in roots and nodules. We hypothesise that gshs1 and gshs2 encode a GSHS and an hGSHS, respectively.
引用
收藏
页码:215 / 219
页数:5
相关论文
共 18 条
[1]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[2]   A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].
DEVEREUX, J ;
HAEBERLI, P ;
SMITHIES, O .
NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395
[3]  
FAHEY RC, 1987, METHOD ENZYMOL, V143, P85
[4]  
FARISS MW, 1987, METHOD ENZYMOL, V143, P101
[5]   A CONSERVED CLEAVAGE-SITE MOTIF IN CHLOROPLAST TRANSIT PEPTIDES [J].
GAVEL, Y ;
VONHEIJNE, G .
FEBS LETTERS, 1990, 261 (02) :455-458
[6]   HOMO-PHYTOCHELATINS ARE HEAVY METAL-BINDING PEPTIDES OF HOMO-GLUTATHIONE CONTAINING FABALES [J].
GRILL, E ;
GEKELER, W ;
WINNACKER, EL ;
ZENK, HH .
FEBS LETTERS, 1986, 205 (01) :47-50
[7]   GAMMA-GLUTAMYLCYSTEINE SYNTHETASE IN HIGHER-PLANTS - CATALYTIC PROPERTIES AND SUBCELLULAR-LOCALIZATION [J].
HELL, R ;
BERGMANN, L .
PLANTA, 1990, 180 (04) :603-612
[8]   PROPERTIES AND LOCALIZATION OF THE HOMOGLUTATHIONE SYNTHETASE FROM PHASEOLUS-COCCINEUS LEAVES [J].
KLAPHECK, S ;
ZOPES, H ;
LEVELS, HG ;
BERGMANN, L .
PHYSIOLOGIA PLANTARUM, 1988, 74 (04) :733-739
[9]   HOMOGLUTATHIONE - ISOLATION, QUANTIFICATION AND OCCURRENCE IN LEGUMES [J].
KLAPHECK, S .
PHYSIOLOGIA PLANTARUM, 1988, 74 (04) :727-732