Self-assembly of cholesterol-hydrotropic dendrimer conjugates into micelle-like structure: Preparation and hydrotropic solubilization of paclitaxel

被引:29
作者
Ooya, T
Huh, KM
Saitoh, M
Tamiya, E
Park, K [1 ]
机构
[1] Purdue Univ, Dept Pharmaceut, W Lafayette, IN 47907 USA
[2] Purdue Univ, Dept Biomed Engn, W Lafayette, IN 47907 USA
[3] Japan Adv Inst Sci & Technol, Sch Math Sci, Nomi, Ishikawa 9231292, Japan
[4] Chungnam Natl Univ, Dept Polymer Sci & Engn, Taejon 305764, South Korea
关键词
dendrimer; hydrotrope; cholesterol; self-assembly; paclitaxel; atomic force microscopy;
D O I
10.1016/j.stam.2005.01.006
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
A hydrotropic dendrimer, made of polyglycerol dendrimer of generation 4 (PGD-G4), was conjugated with cholesterol. The self-assembled structure of the conjugate in water was characterized by H-1-NMR, dynamic light scattering (DLS) and atomic force microscopy (AFM). One cholesterol molecule was conjugated to PGD-G4, which was confirmed by MALDI-TOF mass spectroscopy. The DLS analysis showed that the conjugate formed self-assembly with a diameter of 49.9-59.9 nm. The AFM image suggests that the self-assembly is a micelle-like sphere. The level of paclitaxel solubilization by the conjugate in water was similar to that of PGD-G4 itself, and this suggests that the PGD-G4 is located on the outer part of the self-assembly to function as a hydrotrope. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:452 / 456
页数:5
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