Radiochemical high-performance liquid chromatography detection of arginine metabolism in human endothelial cells

被引：11

作者：

Barilli, Amelia ^{[1
]}

Visigalli, Rossana ^{[1
]}

Rotoli, Bianca Maria ^{[1
]}

Bussolati, Ovidio ^{[1
]}

Gazzola, Gian Carlo ^{[1
]}

Parolari, Alessandro ^{[2
,3
]}

Dall'Asta, Valeria ^{[1
]}

机构：

[1] Univ Parma, Sez Patol Gen & Clin, Dipartimento Med Sperimentale, I-43125 Parma, Italy

[2] Univ Milan, Dipartimento Cardiochirurg, I-20122 Milan, Italy

[3] IRCCS, Ctr Cardiol Fdn Monzino, I-20122 Milan, Italy

来源：

ANALYTICAL BIOCHEMISTRY | 2012年 / 424卷 / 02期

关键词：

Arginine; Nitric oxide; Arginase; Rapamycin; TNF alpha; Phorbol esters; HYDROXY-L-ARGININE; NITRIC-OXIDE; CAT2; TRANSPORTERS; ARGINASE ACTIVITY; STIMULATION; INHIBITION; HEALTH; ALPHA;

D O I：

10.1016/j.ab.2012.02.023

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Arginine is a semi-essential amino acid that plays an important role in the regulation of metabolic processes associated with several pathological/physiological conditions. In the vasculature, it mainly exerts its biological functions as a substrate of two alternative pathways: the conversion to nitric oxide (NO) by nitric oxide synthase (NOS) and the breakdown to urea and ornithine by arginase. To determine arginine metabolism, in the current study we propose an original radiochemical technique that allows the simultaneous monitoring of NOS and arginase activation within intact cells. Taking advantage of this method, we show here the consequences of different experimental conditions known to modulate endothelial homeostasis on arginine metabolism. (c) 2012 Elsevier Inc. All rights reserved.

引用

页码：156 / 161

页数：6

共 16 条

[1]

In human endothelial cells rapamycin causes mTORC2 inhibition and impairs cell viability and function [J].