Mosquito-Bacteria Symbiosis: The Case of Anopheles gambiae and Asaia

被引：117

作者：

Damiani, Claudia ^{[1
]}

Ricci, Irene ^{[1
]}

Crotti, Elena ^{[2
]}

Rossi, Paolo ^{[1
]}

Rizzi, Aurora ^{[2
]}

Scuppa, Patrizia ^{[1
]}

Capone, Aida ^{[1
]}

Ulissi, Ulisse ^{[1
]}

Epis, Sara ^{[1
]}

Genchi, Marco ^{[3
]}

Sagnon, N'Fale ^{[4
]}

Faye, Ingrid ^{[5
]}

Kang, Angray ^{[6
]}

Chouaia, Bessem ^{[2
]}

Whitehorn, Cheryl ^{[7
]}

Moussa, Guelbeogo W. ^{[4
]}

Mandrioli, Mauro ^{[8
]}

Esposito, Fulvio ^{[1
]}

Sacchi, Luciano ^{[3
]}

Bandi, Claudio ^{[9
]}

Daffonchio, Daniele ^{[2
]}

Favia, Guido ^{[1
]}

机构：

[1] Univ Camerino, Scuola Biosci & Biotecnol, I-62032 Camerino, Italy

[2] Univ Milan, Dipartimento Sci & Tecnol Alimentari & Microbiol, I-20133 Milan, Italy

[3] Univ Pavia, Dipartimento Biol Anim, I-27100 Pavia, Italy

[4] CNRFP, Ouagadougou 01, Burkina Faso

[5] Stockholm Univ, Dept Genet Microbiol & Toxicol, SE-10691 Stockholm, Sweden

[6] Univ Westminster, Dept Mol & Appl Biosci, London W1W 6UW, England

[7] London Sch Hyg & Trop Med, Dept Infect & Trop Dis, London WC1 7HT, England

[8] Univ Modena & Reggio Emilia, Dipartimento Biol, I-41125 Modena, Italy

[9] Univ Milan, Dipartimento Patol Anim Igiene & Sanita Pubb Vet, I-20133 Milan, Italy

来源：

MICROBIAL ECOLOGY | 2010年 / 60卷 / 03期

基金：

瑞典研究理事会;

关键词：

GENUS ASAIA; MALARIA; TRANSMISSION; MOLECULES; EXPRESS;

D O I：

10.1007/s00248-010-9704-8

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

The symbiotic relationship between Asaia, an alpha-proteobacterium belonging to the family Acetobacteriaceae, and mosquitoes has been studied mainly in the Asian malaria vector Anopheles stephensi. Thus, we have investigated the nature of the association between Asaia and the major Afro-tropical malaria vector Anopheles gambiae. We have isolated Asaia from different wild and laboratory reared colonies of A. gambiae, and it was detected by PCR in all the developmental stages of the mosquito and in all the specimens analyzed. Additionally, we have shown that it localizes in the midgut, salivary glands and reproductive organs. Using recombinant strains of Asaia expressing fluorescent proteins, we have demonstrated the ability of the bacterium to colonize A. gambiae mosquitoes with a pattern similar to that described for A. stephensi. Finally, fluorescent in situ hybridization on the reproductive tract of females of A. gambiae showed a concentration of Asaia at the very periphery of the eggs, suggesting that transmission of Asaia from mother to offspring is likely mediated by a mechanism of egg-smearing. We suggest that Asaia has potential for use in the paratransgenic control of malaria transmitted by A. gambiae.

引用

页码：644 / 654

页数：11

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