Study of the catalytic effect of copper(II)-protein complexes on luminol-H2O2 chemiluminescence reaction and its analytical application

被引：27

作者：

Li, ZP ^{[1
]}

Li, KA ^{[1
]}

Tong, SY ^{[1
]}

机构：

[1] Beijing Univ, Dept Chem, Beijing 100871, Peoples R China

来源：

ANALYTICAL LETTERS | 1999年 / 32卷 / 05期

基金：

中国国家自然科学基金;

关键词：

protein; copper(II); complex; chemiluminescence; luminol; peroxide;

D O I：

10.1080/00032719908542864

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Through flow injection analysis experiments, it was discovered that unsaturated complexes of Cu(ll) with proteins had a much stronger catalytic effect on the luminol-H2O2 chemiluminescence reaction than did Cu(II). Based on this, a new rapid and sensitive method for determination of some proteins in the range 0.1-20.0 mu g ml(-1) was established. The detection limits were at the 0.03-0.05 mu g ml(-1) level for bovine serum albumin (BSA), human serum albumin (HSA), human gamma-globulin (gamma-G), and egg albumin (EA). The method was used for the determination of proteins in human serum with satisfactory results.

引用

页码：901 / 913

页数：13

共 17 条

[1] FLUOROMETRIC ASSAY OF PROTEINS IN NANOGRAM RANGE [J].

BOHLEN, P ;

STEIN, S ;

DAIRMAN, W ;

UDENFRIEND, S .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1973, 155 (01) :213-220

[2]

BOSTICK DT, 1975, ANAL CHEM, V47, P7

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4] FLOW-INJECTION ANALYSIS FOR MONITORING CHEMI-LUMINESCENT REACTIONS [J].

BURGUERA, JL ;

TOWNSHEND, A ;

GREENFIELD, S .

ANALYTICA CHIMICA ACTA, 1980, 114 (FEB) :209-214

[5]

DOUMAS BT, 1981, CLIN CHEM, V27, P1642

[6] DETERMINATION OF A SMALL AMOUNT OF A BIOLOGICAL CONSTITUENT BY THE USE OF CHEMI-LUMINESCENCE .2. DETERMINATION OF ALBUMIN AS A MODEL PROTEIN BY MEANS OF THE FLOW-INJECTION ANALYSIS USING A COBALT(III) COMPLEX COMPOUND AS A CATALYST [J].

HARA, T ;

TORIYAMA, M ;

TSUKAGOSHI, K .

BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 1984, 57 (01) :289-290

[7] DETERMINATION OF A SMALL AMOUNT OF BIOLOGICAL CONSTITUENT BY USE OF CHEMI-LUMINESCENCE .3. THE FLOW-INJECTION ANALYSIS OF PROTEIN BY DIRECT INJECTION [J].

HARA, T ;

TORIYAMA, M ;

TSUKAGOSHI, K .

BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 1984, 57 (06) :1551-1555

[8] DETERMINATION OF A SMALL AMOUNT OF BIOLOGICAL CONSTITUENT BY USE OF CHEMI-LUMINESCENCE .1. THE FLOW-INJECTION ANALYSIS OF PROTEIN [J].

HARA, T ;

TORIYAMA, M ;

TSUKAGOSHI, K .

BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 1983, 56 (05) :1382-1387

[9] THE DETERMINATION OF A SMALL AMOUNT OF BIOLOGICAL CONSTITUENT BY THE USE OF CHEMI-LUMINESCENCE .4. A HIGHLY SENSITIVE DETECTOR FOR THE HPLC OF PROTEIN [J].

HARA, T ;

TORIYAMA, M ;

EBUCHI, T .

BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 1985, 58 (01) :109-114

[10] Fluorometric determination for micro amounts of albumin and globulin fractions without separation by using alpha,beta,gamma,delta-tetra(4'-carboxyphenyl)porphin [J].

Li, N ;

Li, KA ;

Tong, SY .

ANALYTICAL BIOCHEMISTRY, 1996, 233 (02) :151-155

← 1 2 →