Methods to determine biotin-binding capacity of streptavidin-coated magnetic particles

被引：16

作者：

Dorgan, L ^{[1
]}

Magnotti, R ^{[1
]}

Hou, JM ^{[1
]}

Engle, T ^{[1
]}

Ruley, K ^{[1
]}

Shull, B ^{[1
]}

机构：

[1] Seradyn, Particle Technol Div, Indianapolis, IN 46225 USA

来源：

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS | 1999年 / 194卷 / 1-3期

关键词：

magnetic microparticles; streptavidin; biotin binding;

D O I：

10.1016/S0304-8853(98)00563-0

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Two assays to determine the biotin-binding capacity of streptavidin magnetic particles are described and compared. The two assays are based on the use of biotinylated alkaline phosphatase and biotinylated fluorescein, respectively. Also, an assay for bound protein is presented. When the biotin-binding methods are combined with the protein assay, the specific activity can be determined. The fluorescent version is used to compare the streptavidin magnetic particles from several manufacturers. (C) 1999 Published by Elsevier Science B.V. All rights reserved.

引用

页码：69 / 75

页数：7