Gαolf is necessary for coupling D1 and A2a receptors to adenylyl cyclase in the striatum

被引:162
作者
Corvol, JC
Studler, JM
Schonn, JS
Girault, JA
Hervé, D
机构
[1] Inst Fre Moulin, INSERM U536, F-75005 Paris, France
[2] Coll France, INSERM U114, F-75231 Paris, France
关键词
A2a receptor; adenylyl cyclase; D1; receptor; G alpha(olf); G alpha(s); striatum;
D O I
10.1046/j.1471-4159.2001.00201.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In the brain, dopamine and adenosine stimulate cyclic AMP (cAMP) production through D1 and A2a receptors, respectively. Using mutant mice deficient in the olfactory isoform of the stimulatory GTP-binding protein alpha subunit, G alpha (olf), we demonstrate here the obligatory role of this protein in the adenylyl cyclase responses to dopamine and adenosine in the caudate putamen. Responses to dopamine were also dramatically decreased in the nucleus accumbens but remained unaffected in the prefrontal cortex. Moreover, in the caudate putamen of mice heterozygous for the mutation, the amounts of G alpha (olf) were half of the normal levels, and the efficacy of dopamine- and CGS 21680 A(2) agonist-stimulated cAMP production was decreased. Together, these results identify G alpha (olf) as a critical parameter in the responses to dopamine and adenosine in the basal ganglia.
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页码:1585 / 1588
页数:4
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