Genome-wide RNAi analysis of growth and viability in Drosophila cells

被引：551

作者：

Boutros, M

Kiger, AA

Armknecht, S

Kerr, K

Hild, M

Koch, B

Haas, SA

Paro, R

Perrimon, N ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Howard Hughes Med Inst, Boston, MA 02115 USA

[3] Heidelberg Univ, Zentrum Mol Biol, D-69120 Heidelberg, Germany

[4] Max Planck Inst Mol Genet, D-14195 Berlin, Germany

来源：

SCIENCE | 2004年 / 303卷 / 5659期

关键词：

D O I：

10.1126/science.1091266

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A crucial aim upon completion of whole genome sequences is the functional analysis of all predicted genes. We have applied a high-throughput RNA-interference (RNAi) screen of 19,470 double-stranded (ds) RNAs in cultured cells to characterize the function of nearly all (91%) predicted Drosophila genes in cell growth and viability. We found 438 dsRNAs that identified essential genes, among which 80% lacked mutant alleles. A quantitative assay of cell number was applied to identify genes of known and uncharacterized functions. In particular, we demonstrate a role for the homolog of a mammalian acute myeloid leukemia gene (AML1) in cell survival. Such a systematic screen for cell phenotypes, such as cell viability, can thus be effective in characterizing functionally related genes on a genome-wide scale.

引用

页码：832 / 835

页数：4

共 21 条

[1] Comparison of the complete protein sets of worm and yeast: Orthology and divergence
Chervitz, SA
Aravind, L
Sherlock, G
Ball, CA
Koonin, EV
Dwight, SS
Harris, MA
Dolinski, K
Mohr, S
Smith, T
Weng, S
Cherry, JM
Botstein, D
[J]. SCIENCE, 1998, 282 (5396) : 2022 - 2028
[2] Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways
Clemens, JC
Worby, CA
Simonson-Leff, N
Muda, M
Maehama, T
Hemmings, BA
Dixon, JE
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (12) : 6499 - 6503
[3] ECHALIER G, 1970, IN VITRO CELL DEV B, V6, P162
[4] Frank LH, 1996, DEVELOPMENT, V122, P1343
[5] The FlyBase database of the Drosophila genome projects and community literature
Gelbart, W
Bayraktaroglu, L
Bettencourt, B
Campbell, K
Crosby, M
Emmert, D
Hradecky, P
Huang, Y
Letovsky, S
Matthews, B
Russo, S
Schroeder, A
Smutniak, F
Zhou, P
Zytkovicz, M
Ashburner, M
Drysdale, R
de Grey, A
Foulger, R
Millburn, G
Yamada, C
Kaufman, T
Matthews, K
Gilbert, D
Grumbling, G
Strelets, V
Shemen, C
Rubin, G
Berman, B
Frise, E
Gibson, M
Harris, N
Kaminker, J
Lewis, S
Marshall, B
Misra, S
Mungall, C
Prochnik, S
Richter, J
Smith, C
Shu, S
Tupy, J
Wiel, C
[J]. NUCLEIC ACIDS RESEARCH, 2003, 31 (01) : 172 - 175
[6] Functional profiling of the Saccharomyces cerevisiae genome
Giaever, G
Chu, AM
Ni, L
Connelly, C
Riles, L
Véronneau, S
Dow, S
Lucau-Danila, A
Anderson, K
André, B
Arkin, AP
Astromoff, A
El Bakkoury, M
Bangham, R
Benito, R
Brachat, S
Campanaro, S
Curtiss, M
Davis, K
Deutschbauer, A
Entian, KD
Flaherty, P
Foury, F
Garfinkel, DJ
Gerstein, M
Gotte, D
Güldener, U
Hegemann, JH
Hempel, S
Herman, Z
Jaramillo, DF
Kelly, DE
Kelly, SL
Kötter, P
LaBonte, D
Lamb, DC
Lan, N
Liang, H
Liao, H
Liu, L
Luo, CY
Lussier, M
Mao, R
Menard, P
Ooi, SL
Revuelta, JL
Roberts, CJ
Rose, M
Ross-Macdonald, P
Scherens, B
[J]. NATURE, 2002, 418 (6896) : 387 - 391
[7] Molecular classification of cancer: Class discovery and class prediction by gene expression monitoring
Golub, TR
Slonim, DK
Tamayo, P
Huard, C
Gaasenbeek, M
Mesirov, JP
Coller, H
Loh, ML
Downing, JR
Caligiuri, MA
Bloomfield, CD
Lander, ES
[J]. SCIENCE, 1999, 286 (5439) : 531 - 537
[8] Cell proliferation and apoptosis
Guo, M
Hay, BA
[J]. CURRENT OPINION IN CELL BIOLOGY, 1999, 11 (06) : 745 - 752
[9] Drosophila homologs of baculovirus inhibitor of apoptosis proteins function to block cell death
Hay, BA
Wassarman, DA
Rubin, GM
[J]. CELL, 1995, 83 (07) : 1253 - 1262
[10] Hild M, 2004, GENOME BIOL, V5

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