Evaluation of flavonoids from Bauhinia megalandra leaves as inhibitors of glucose-6-phosphatase system

被引：37

作者：

Estrada, O

Hasegawa, M

Gonzalez-Mujíca, F

Motta, N

Perdomo, E

Solorzano, A

Méndez, J

Méndez, B

Zea, EG

机构：

[1] Cent Univ Venezuela, Fac Ciencias, Escuela Quim, Ctr Quim Organ, Caracas, Venezuela

[2] Cent Univ Venezuela, Fac Med, Inst Expt Med, Secc Bioquim Med, Sabana Grande, Venezuela

来源：

PHYTOTHERAPY RESEARCH | 2005年 / 19卷 / 10期

关键词：

Bauhinia megalandra; flavonoids; glucose-6-phosphatase; diabetes;

D O I：

10.1002/ptr.1703

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

From the methanol extract of Bauhinia megalandra fresh leaves, eight flavonoids were isolated and evaluated by rat liver microsomal glucose-6-phosphatase (G-6-Pase) bioassay, which might be a useful methodology for screening antihyperglycaemic substances. All the flavonoids assayed showed an inhibitory effect on the intact microsomal G-6-Pase: quercetin and kaempferol exhibited the lowest effect; astilbin, quercetin 3-O-alpha-rhamnoside, kaempferol 3-O-alpha-rhamnoside and quercetin 3-O-alpha-arabinoside an intermediate effect. The highest inhibitory activity was shown by quercetin 3-O-alpha-(2 ''-galloyl)rhamnoside and kaempferol 3-O-alpha-(2 '' galloyl)rhamnoside. None of the flavonoids mentioned above showed an inhibitory effect on the disrupted microsomal G-6-Pase. Quercetin 3-O-alpha-(2 ''-galloyl)rhamnoside and kaempferol 3-O-alpha-(2 ''-galloyl)rhamnoside exhibited the lowest IC50 of all the flavonoids assayed. Also, the phlorizin IC50 is reported. Copyright (c) 2005 John Wiley & Sons, Ltd.

引用

页码：859 / 863

页数：5

共 22 条

[1]

Agrawal P.K., 1989, Carbon-13 NMR of Flavonoids

[2] Direct evidence for the involvement of two glucose 6-phosphate-binding sites in the glucose-6-phosphatase activity of intact liver microsomes - Characterization of T1, the microsomal glucose 6-phosphate transport protein by a direct binding assay [J].