MicroRNA-124 Is a Subventricular Zone Neuronal Fate Determinant

被引：179

作者：

Akerblom, Malin ^{[1
,2
]}

Sachdeva, Rohit ^{[1
,2
]}

Barde, Isabelle ^{[3
]}

Verp, Sonia ^{[3
]}

Gentner, Bernhard ^{[4
]}

Trono, Didier ^{[3
]}

Jakobsson, Johan ^{[1
,2
]}

机构：

[1] Lund Univ, Wallenberg Neurosci Ctr, Dept Expt Med Sci, S-22184 Lund, Sweden

[2] Lund Univ, Lund Stem Cell Ctr, S-22184 Lund, Sweden

[3] Ecole Polytech Fed Lausanne, Sch Life Sci, CH-1015 Lausanne, Switzerland

[4] Univ Vita Salute San Raffaele, San Raffaele Telethon Inst Gene Therapy, I-20132 Milan, Italy

来源：

JOURNAL OF NEUROSCIENCE | 2012年 / 32卷 / 26期

基金：

瑞典研究理事会;

关键词：

NEURAL STEM-CELLS; ADULT-MOUSE BRAIN; LENTIVIRAL VECTORS; IN-VIVO; HUMAN FIBROBLASTS; MIR-124; IDENTIFICATION; EXPRESSION; DIFFERENTIATION; CULTURES;

D O I：

10.1523/JNEUROSCI.0558-12.2012

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

New neurons are continuously generated from neural stem cells with astrocyte properties, which reside in close proximity to the ventricle in the postnatal and adult brain. In this study we found that microRNA-124 (miR-124) dictates postnatal neurogenesis in the mouse subventricular zone. Using a transgenic reporter mouse we show that miR-124 expression is initiated in the rapid amplifying progenitors and remains expressed in the resulting neurons. When we stably inhibited miR-124 in vivo, neurogenesis was blocked, leading to the appearance of ectopic cells with astrocyte characteristics in the olfactory bulb. Conversely, when we overexpressed miR-124, neural stem cells were not maintained in the subventricular zone and neurogenesis was lost. In summary, our results demonstrate that miR-124 is a neuronal fate determinant in the subventricular zone.

引用

页码：8879 / 8889

页数：11

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