A novel transcriptional coactivator, p52, functionally interacts with the essential splicing factor ASF/SF2

被引：118

作者：

Ge, H ^{[1
]}

Si, YZ ^{[1
]}

Wolffe, AP ^{[1
]}

机构：

[1] NICHHD, Mol Embryol Lab, NIH, Bethesda, MD 20892 USA

来源：

MOLECULAR CELL | 1998年 / 2卷 / 06期

关键词：

D O I：

10.1016/S1097-2765(00)80290-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Increasing evidence suggests that pre-mRNA splicing can take place cotranscriptionally in vivo. However, insight into how these two processes are linked has been lacking, Here, we describe that a novel transcriptional coactivator, p52, interacts not only with transcriptional activators and general transcription Factors to enhance activated transcription but also with the essential splicing factor ASF/SF2 both in vitro and in vivo to modulate ASF/SF2-mediated pre-mRNA splicing. Furthermore, immunofluorescence studies indicate that the majority of endogenous p52 is colocalized with ASF/SF2 in the nucleoplasm of HeLa cells. Together, these observations suggest that, in addition to functioning as a transcriptional coactivator, p52 may also act as an adaptor to coordinate pre-mRNA splicing and transcriptional activation of class ii genes.

引用

页码：751 / 759

页数：9

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