Three-Dimensional Hybridization with polyvalent DNA-gold nanoparticle conjugates

被引:88
作者
Hurst, Sarah J.
Hill, Haley D.
Mirkin, Chad A. [1 ]
机构
[1] Northwestern Univ, Dept Chem, Evanston, IL 60208 USA
关键词
D O I
10.1021/ja804266j
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We have determined the minimum number of base pairings necessary to stabilize DNA-Au NP aggregates as a function of salt concentration for particles between 15 and 150 nm in diameter. Significantly, we find that sequences containing a single base pair interaction are capable of effecting hybridization between 150 nm DNA-Au NPs. While traditional DNA hybridization involves two strands interacting in one dimension (1D, Z), we propose that hybridization in the context of an aggregate of polyvalent DNA-Au NP conjugates occurs in three dimensions (many oligonucleotides oriented perpendicular to the X, Y plane engage in base pairing), making nanoparticle assembly possible with three or fewer base pairings per DNA strand. These studies enabled us to compare the stability of duplex DNA free in solution and bound to the nanoparticle surface. We estimate that 4-8, 6-19, or 8-33 additional DNA bases must be added to free duplex DNA to achieve melting temperatures equivalent to hybridized systems formed from 15, 60, or 150 nm DNA-Au NPs, respectively. In addition, we estimate that the equilibrium binding constant (K-eq) for 15 nm DNA-Au NIPS (3 base pairs) is similar to 3 orders of magnitude higher than the K-eq for the corresponding nanoparticle free system.
引用
收藏
页码:12192 / 12200
页数:9
相关论文
共 29 条
[1]   Organization of 'nanocrystal molecules' using DNA [J].
Alivisatos, AP ;
Johnsson, KP ;
Peng, XG ;
Wilson, TE ;
Loweth, CJ ;
Bruchez, MP ;
Schultz, PG .
NATURE, 1996, 382 (6592) :609-611
[2]   Optical absorption spectra of nanocrystal gold molecules [J].
Alvarez, MM ;
Khoury, JT ;
Schaaff, TG ;
Shafigullin, MN ;
Vezmar, I ;
Whetten, RL .
JOURNAL OF PHYSICAL CHEMISTRY B, 1997, 101 (19) :3706-3712
[3]  
Bloomfield VA, 2000, NUCL ACIDS STRUCTURE
[4]  
Clint J.H., 1992, Surfactant Aggregation
[5]   A fluorescence-based method for determining the surface coverage and hybridization efficiency of thiol-capped oligonucleotides bound to gold thin films and nanoparticles [J].
Demers, LM ;
Mirkin, CA ;
Mucic, RC ;
Reynolds, RA ;
Letsinger, RL ;
Elghanian, R ;
Viswanadham, G .
ANALYTICAL CHEMISTRY, 2000, 72 (22) :5535-5541
[6]   Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles [J].
Elghanian, R ;
Storhoff, JJ ;
Mucic, RC ;
Letsinger, RL ;
Mirkin, CA .
SCIENCE, 1997, 277 (5329) :1078-1081
[7]   Maximizing DNA loading on a range of gold nanoparticle sizes [J].
Hurst, Sarah J. ;
Lytton-Jean, Abigail K. R. ;
Mirkin, Chad A. .
ANALYTICAL CHEMISTRY, 2006, 78 (24) :8313-8318
[8]   What controls the melting properties of DNA-linked gold nanoparticle assemblies? [J].
Jin, RC ;
Wu, GS ;
Li, Z ;
Mirkin, CA ;
Schatz, GC .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (06) :1643-1654
[9]   Synthetic multivalent ligands in the exploration of cell-surface interactions [J].
Kiessling, LL ;
Gestwicki, JE ;
Strong, LE .
CURRENT OPINION IN CHEMICAL BIOLOGY, 2000, 4 (06) :696-703
[10]   DNA-induced size-selective separation of mixtures of gold nanoparticles [J].
Lee, Jae-Seung ;
Stoeva, Savka I. ;
Mirkin, Chad A. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (27) :8899-8903