Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex

被引：41

作者：

Debler, Erik W. ^{[2
,3
]}

Kaufmann, Gunnar F. ^{[3
,4
,5
]}

Meijler, Michael M. ^{[3
,4
]}

Heine, Andreas ^{[2
]}

Mee, Jenny M. ^{[3
,4
]}

Pljevaljcic, Goran ^{[2
]}

Di Bilio, Angel J. ^{[1
]}

Schultz, Peter G. ^{[3
,4
]}

Millar, David P. ^{[2
]}

Janda, Kim D. ^{[3
,4
,5
,6
]}

Wilson, Ian A. ^{[2
,3
]}

Gray, Harry B. ^{[1
]}

Lerner, Richard A. ^{[2
,3
,4
]}

机构：

[1] CALTECH, Beckman Inst, Pasadena, CA 91125 USA

[2] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

[3] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

[4] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[5] Scripps Res Inst, Dept Immunol, La Jolla, CA 92037 USA

[6] Scripps Res Inst, Worm Inst Res & Med, La Jolla, CA 92037 USA

来源：

SCIENCE | 2008年 / 319卷 / 5867期

关键词：

D O I：

10.1126/science.1153445

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The blue- emissive antibody EP2-19G2 that has been elicited against trans- stilbene has unprecedented ability to produce bright luminescence and has been used as a biosensor in various applications. We show that the prolonged luminescence is not stilbene fluorescence. Instead, the emissive species is a charge- transfer excited complex of an anionic stilbene and a cationic, parallel pi-stacked tryptophan. Upon charge recombination, this complex generates exceptionally bright blue light. Complex formation is enabled by a deeply penetrating ligand-binding pocket, which in turn results from a noncanonical interface between the two variable domains of the antibody.

引用

页码：1232 / 1235

页数：4

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