Reproductive success and biochemical effects in tree swallows (Tachycineta bicolor) exposed to chlorinated hydrocarbon contaminants in wetlands of the Great Lakes and St. Lawrence River basin, USA and Canada

Reproduction and biochemical indicators (vitamin A, ethoxyresorufin-O-deethylase [EROD], highly carboxylated porphyrins) of contaminant effects, and concentrations of organochlorine residues were measured in tree swallows from wetlands within the watersheds of the Great Lakes and the St. Lawrence River, USA and Canada. To assess the vitamin A sources in the tree swallow diets, insects were collected, by ligature, from nestlings at four of these sites. Diet samples were analyzed for retinol and alpha- and beta-carotene. Marked differences were found in chlorinated hydrocarbon residues in eggs and nestlings among sites; however, we did not find any significant differences in hatching or hedging success. The maximum polychlorinated biphenyl (PCB) concentration found was 11.1 mu g/g in eggs from Akwesasne Reserve, New York, USA, on the St. Lawrence River, whereas the highest p,p'-DDE concentration of 2.57 mu g/g was found in eggs from Mud Creek, Ontario, Canada, in the Lake Erie watershed. Concentrations of other organochlorine pesticides and chlorobenzenes were low and not variable among sites. Significant differences in hepatic EROD activity, retinol and retinyl palmitate, and uroporphyrin in nestling birds existed among sites. Ethoxyresorufin O-deethylase activity was highest and retinol and retinyl palmitate were lowest at Cornwall Island, Ontario, Canada, in the St. Lawrence River, whereas porphyrins were highest at Toronto and Hamilton harbors, Ontario, Canada, in Lake Ontario. Ethoxyresorufin O-deethylase induction was significantly and negatively correlated with nestling hepatic retinol concentrations, whereas uroporphyrin concentrations were positively correlated with PCB congener 118 in nestlings. In contrast, few differences occurred in vitamin A concentrations in diet samples among sites. Together, these findings suggest that depressed hepatic vitamin A and elevated highly carboxylated porphyrin concentrations are strongly associated with sites with the highest organochlorine exposure in tree swallows.