Purification of a pluripotent neural stem cell from the adult mouse brain

被引:514
作者
Rietze, RL
Valcanis, H
Brooker, GF
Thomas, T
Voss, AK
Bartlett, PF
机构
[1] Walter & Eliza Hall Inst Med Res, Parkville, Vic 3050, Australia
[2] Univ Melbourne, Howard Florey Inst Expt Physiol & Med, Parkville, Vic 3010, Australia
关键词
D O I
10.1038/35089085
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The adult mammalian central nervous system (CNS) contains a population of neural stem cells (NSCs)(1-4) with properties said to include the generation of non-neural progeny(5-7). However, the precise identity, location and potential of the NSC in situ remain unclear. We purified NSCs from the adult mouse brain by flow cytometry, and directly examined the cells' properties. Here we show that one type of NSC, which expresses the protein nestin but only low levels of PNA-binding and HSA proteins, is found in both ependymal and subventricular zones and accounts for about 63% of the total NSC activity. Furthermore, the selective depletion of the population of this stem cell in querkopf(8) mutant mice (which are deficient in production of olfactory neurons) suggests that it acts as a major functional stem cell in vivo. Most freshly isolated NSCs, when co-cultured with a muscle cell line, rapidly differentiated in vitro into myocytes that contain myosin heavy chain (MyHC). This demonstrates that a predominant, functional type of stem cell exists in the periventricular region of the adult brain with the intrinsic ability to generate neural and non-neural cells.
引用
收藏
页码:736 / 739
页数:4
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