A reverse dot-blot method for rapid detection of non-deletion α thalassaemia

被引：51

作者：

Chan, V ^{[1
]}

Yam, I ^{[1
]}

Chen, FE ^{[1
]}

Chan, TK ^{[1
]}

机构：

[1] Univ Hong Kong, Queen Mary Hosp, Dept Med, Hong Kong, Peoples R China

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 1999年 / 104卷 / 03期

关键词：

reverse dot blot; allele-specific hybridization; non-deletion defects deletion alpha thalassaemia;

D O I：

10.1046/j.1365-2141.1999.01221.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A reverse dot blot method based on membrane-bound allele-specific oligonucleotides as hybridization targets for amplified alpha-gene fragments has been developed for the rapid detection of four non-deletion alpha thalassaemia defects found in the Chinese. Since these non-deletion defects account for 22.8% of haemoglobin H disease, a sensitive, specific and rapid screening method should be of value.

引用

页码：513 / 515

页数：3

共 11 条

[1] PRENATAL-DIAGNOSIS OF HOMOZYGOUS ALPHA-THALASSEMIA BY DIRECT DNA ANALYSIS OF UNCULTURED AMNIOTIC-FLUID CELLS [J].

CHAN, V ;

GHOSH, A ;

CHAN, TK ;

WONG, V ;

TODD, D .

BMJ-BRITISH MEDICAL JOURNAL, 1984, 288 (6427) :1327-1329

[2] Molecular defects in Hb H hydrops fetalis [J].

Chan, V ;

Chan, VWY ;

Tang, M ;

Lau, K ;

Todd, D .

BRITISH JOURNAL OF HAEMATOLOGY, 1997, 96 (02) :224-228

[3]

CHAN V, 1985, BLOOD, V66, P224

[4] MOLECULAR DIAGNOSTICS - PAST, PRESENT, AND FUTURE [J].

CHEHAB, FF .

HUMAN MUTATION, 1993, 2 (05) :331-337

[5]

Chim CS, 1998, AM J HEMATOL, V57, P160, DOI 10.1002/(SICI)1096-8652(199802)57:2<160::AID-AJH12>3.0.CO

[6]

2-G

[7] LOCUS ASSIGNMENT OF HUMAN ALPHA-GLOBIN MUTATIONS BY SELECTIVE AMPLIFICATION AND DIRECT SEQUENCING [J].

DODE, C ;

ROCHETTE, J ;

KRISHNAMOORTHY, R .

BRITISH JOURNAL OF HAEMATOLOGY, 1990, 76 (02) :275-281

[8]

HALL GW, 1993, BRIT J HAEMATOL, V85, P546

[9] MOLECULAR CHARACTERIZATION OF SEVERE ALPHA-THALASSEMIAS CAUSING HYDROPS-FETALIS IN TAIWAN [J].

KO, TM ;

HSIEH, FJ ;

HSU, PM ;

LEE, TY .

AMERICAN JOURNAL OF MEDICAL GENETICS, 1991, 39 (03) :317-320

[10] GENETIC-ANALYSIS OF AMPLIFIED DNA WITH IMMOBILIZED SEQUENCE-SPECIFIC OLIGONUCLEOTIDE PROBES [J].

SAIKI, RK ;

WALSH, PS ;

LEVENSON, CH ;

ERLICH, HA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (16) :6230-6234

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