Control of glucose- and NaCI-induced biofilm formation by rbf in Staphylococcus aureus

被引:149
作者
Lim, Y [1 ]
Jana, M [1 ]
Luong, TT [1 ]
Lee, CY [1 ]
机构
[1] Univ Kansas, Med Ctr, Dept Microbiol Mol Genet & Immunol, Kansas City, KS 66160 USA
关键词
D O I
10.1128/JB.186.3.722-729.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Both Staphylococcus aureus and S. epidermidis are capable of forming biofilm on biomaterials. We used Tn917 mutagenesis to identify a gene, rbf, affecting biofilm formation in S. aureus NCTC8325-4. Sequencing revealed that Rbf contained a consensus region signature of the AraC/XylS family of regulators, suggesting that Rbf is a transcriptional regulator. Insertional duplication inactivation of the rbf gene confirmed that the gene was involved in biofilm formation on polystyrene and glass. Phenotypic analysis of the wild type and the mutant suggested that the rbf gene mediates the biofilm formation of S. aureus at the multicellular aggregation stage rather than at initial attachment. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis demonstrated that the mutation resulted in the loss of an similar to190-kDa protein. Biofilm production by the mutant could be restored by complementation with a 2.5-kb DNA fragment containing the rbf gene. The rbf-specific mutation affected the induction of biofilm formation by glucose and a high concentration of NaCl but not by ethanol. The mutation did not affect the transcription of the ica genes previously shown to be required for biofilm formation. Taken together, our results suggest that the rbf gene is involved in the regulation of the multicellular aggregation step of S. aureus biofilm formation in response to glucose and salt and that this regulation may be mediated through the 190-kDa protein.
引用
收藏
页码:722 / 729
页数:8
相关论文
共 43 条
[1]   Mutation of sarA in Staphylococcus aureus limits biofilm formation [J].
Beenken, KE ;
Blevins, JS ;
Smeltzer, MS .
INFECTION AND IMMUNITY, 2003, 71 (07) :4206-4211
[2]   Alpha-toxin is required for biofilm formation by Staphylococcus aureus [J].
Caiazza, NC ;
O'Toole, GA .
JOURNAL OF BACTERIOLOGY, 2003, 185 (10) :3214-3217
[3]   icaR encodes a transcriptional repressor involved in environmental regulation of ica operon expression and biofilm formation in Staphylococcus epidermidis [J].
Conlon, KM ;
Humphreys, H ;
O'Gara, JP .
JOURNAL OF BACTERIOLOGY, 2002, 184 (16) :4400-4408
[4]   Bacterial biofilms: A common cause of persistent infections [J].
Costerton, JW ;
Stewart, PS ;
Greenberg, EP .
SCIENCE, 1999, 284 (5418) :1318-1322
[5]   The intercellular adhesion (ica) locus is present in Staphylococcus aureus and is required for biofilm formation [J].
Cramton, SE ;
Gerke, C ;
Schnell, NF ;
Nichols, WW ;
Götz, F .
INFECTION AND IMMUNITY, 1999, 67 (10) :5427-5433
[6]   Bap, a Staphylococcus aureus surface protein involved in biofilm formation [J].
Cucarella, C ;
Solano, C ;
Valle, J ;
Amorena, B ;
Lasa, I ;
Penadés, JR .
JOURNAL OF BACTERIOLOGY, 2001, 183 (09) :2888-2896
[7]  
DOBINSKY S, J BACTERIOL, V185, P2879
[8]   AraC/XylS family of transcriptional regulators [J].
Gallegos, MT ;
Schleif, R ;
Bairoch, A ;
Hofmann, K ;
Ramos, JL .
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1997, 61 (04) :393-+
[9]   Staphylococcus and biofilms [J].
Götz, F .
MOLECULAR MICROBIOLOGY, 2002, 43 (06) :1367-1378
[10]  
HEILMAN C, 1997, MOL MICROBIOL, V29, P1013