Intraflagellar transport (IFT) cargo: IFT transports flagellar precursors to the tip and turnover products to the cell body

被引：278

作者：

Qin, HM ^{[1
]}

Diener, DR ^{[1
]}

Geimer, S ^{[1
]}

Cole, DG ^{[1
]}

Rosenbaum, JL ^{[1
]}

机构：

[1] Yale Univ, MCDB Dept, New Haven, CT 06520 USA

来源：

JOURNAL OF CELL BIOLOGY | 2004年 / 164卷 / 02期

关键词：

cilia; intraflagellar; transport; kinesin; radial spoke;

D O I：

10.1083/jcb.200308132

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Intraflagellar transport (IFT) is the bidirectional movement of multisubunit protein particles along axonemal microtubules and is required for assembly and maintenance of eukaryotic flagella and cilia. One posited role of IFT is to transport flagellar precursors to the flagellar tip for assembly. Here, we examine radial spokes, axonemal subunits consisting of 22 polypeptides, as potential cargo for IFT. Radial spokes were found to be partially assembled in the cell body, before being transported to the flagellar tip by anterograde IFT. Fully assembled radial spokes, detached from axonemal microtubules during flagellar breakdown or turnover, are removed from flagella by retrograde IFT. Interactions between IFT particles, motors, radial spokes, and other axonemal proteins were verified by coimmuno-precipitation of these proteins from the soluble fraction of Chlamydomonas flagella. These studies indicate that one of the main roles of IFT in flagellar assembly and maintenance is, to transport axonemal proteins in and out of the flagellum.

引用

页码：255 / 266

页数：12

共 57 条

[1]

Bell SE, 2001, J CELL SCI, V114, P2755

[2] Light chain 1 from the Chlamydomonas outer dynein arm is a leucine-rich repeat protein associated with the motor domain of the γ heavy chain [J].