Differential transcriptional analysis of the cyanobacterium Cyanothece sp strain ATCC 51142 during light-dark and continuous-light growth

被引:110
作者
Toepel, Joerg [1 ]
Welsh, Eric
Summerfield, Tina C. [1 ]
Pakrasi, Himadri B. [2 ]
Sherman, Louis A. [1 ]
机构
[1] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA
[2] Washington Univ, Dept Biol, St Louis, MO 63130 USA
关键词
D O I
10.1128/JB.00206-08
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We analyzed the metabolic rhythms and differential gene expression in the unicellular, diazotrophic cyanobacterium Cyanothece sp. strain ATCC 51142 under N-2-fixing conditions after a shift from normal 12-h light-12-h dark cycles to continuous light. We found that the mRNA levels of similar to 10% of the genes in the genome demonstrated circadian behavior during growth in free-running (continuous light) conditions. The genes for N-2 fixation displayed a strong circadian behavior, whereas photosynthesis and respiration genes were not as tightly regulated. One of our main objectives was to determine the strategies used by these cells to perform N-2 fixation under normal day-night conditions, as well as under the greater stress caused by continuous light. We determined that N-2 fixation cycled in continuous light but with a lower N-2 fixation activity. Glycogen degradation, respiration, and photosynthesis were also lower; nonetheless, O-2 evolution was about 50% of the normal peak. We also demonstrated that nifH (encoding the nitrogenase Fe protein), nijB, and nifX were strongly induced in continuous light; this is consistent with the role of these proteins during the assembly of the enzyme complex and suggested that the decreased N-2 fixation activity was due to protein-level regulation or inhibition. Many soluble electron carriers (e.g., ferredoxins), as well as redox carriers (e.g., thioredoxin and glutathione), were strongly induced during N-2 fixation in continuous light. We suggest that these carriers are required to enhance cyclic electron transport and phosphorylation for energy production and to maintain appropriate redox levels in the presence of elevated O-2, respectively.
引用
收藏
页码:3904 / 3913
页数:10
相关论文
共 53 条
  • [1] Involvement of NtcB, a LysR family transcription factor, in nitrite activation of the nitrate assimilation operon in the cyanobacterium Synechococcus sp. strain PCC 7942
    Aichi, M
    Omata, T
    [J]. JOURNAL OF BACTERIOLOGY, 1997, 179 (15) : 4671 - 4675
  • [2] Role of NtcB in activation of nitrate assimilation genes in the cyanobacterium Synechocystis sp strain PCC 6803
    Aichi, M
    Takatani, N
    Omata, T
    [J]. JOURNAL OF BACTERIOLOGY, 2001, 183 (20) : 5840 - 5847
  • [3] Segregation of nitrogen fixation and oxygenic photosynthesis in the marine cyanobacterium Trichodesmium
    Berman-Frank, I
    Lundgren, P
    Chen, YB
    Küpper, H
    Kolber, Z
    Bergman, B
    Falkowski, P
    [J]. SCIENCE, 2001, 294 (5546) : 1534 - 1537
  • [4] Cloning, sequencing, and regulation of the global nitrogen regulator gene ntcA in the unicellular diazotrophic cyanobacterium Cyanothece sp. strain BH68K
    Bradley, RL
    Reddy, KJ
    [J]. JOURNAL OF BACTERIOLOGY, 1997, 179 (13) : 4407 - 4410
  • [5] Kinetic and structural analysis of the ultrasensitive behaviour of cyanobacterial ADP-glucose pyrophosphorylase
    Casati, DFG
    Aon, MA
    Iglesias, AA
    [J]. BIOCHEMICAL JOURNAL, 2000, 350 : 139 - 147
  • [6] Expression of photosynthesis genes in relation to nitrogen fixation in the diazotrophic filamentous nonheterocystous cyanobacterium Trichodesmium sp IMS 101
    Chen, YB
    Dominic, B
    Zani, S
    Mellon, MT
    Zehr, JP
    [J]. PLANT MOLECULAR BIOLOGY, 1999, 41 (01) : 89 - 104
  • [7] Temporal patterns of nitrogenase gene (nifH) expression in the oligotrophic North Pacific Ocean
    Church, MJ
    Short, CM
    Jenkins, BD
    Karl, DM
    Zehr, JP
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (09) : 5362 - 5370
  • [8] Colón-López MS, 1998, J BACTERIOL, V180, P519
  • [9] Transcriptional and translational regulation of nitrogenase in light-dark- and continuous-light grown cultures of the unicellular cyanobacterium Cyanothece sp. strain ATCC 51142
    ColonLopez, M
    Sherman, DM
    Sherman, LA
    [J]. JOURNAL OF BACTERIOLOGY, 1997, 179 (13) : 4319 - 4327
  • [10] NifB-dependent in vitro synthesis of the iron-molybdenum cofactor of nitrogenase
    Curatti, L
    Ludden, PW
    Rubio, LM
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (14) : 5297 - 5301