Isolation and characterization of a novel gene close to the human phosphoinositide-specific phospholipase C beta 3 gene on chromosomal region 11q13

被引：21

作者：

Lagercrantz, J ^{[1
]}

Carson, E ^{[1
]}

Larsson, C ^{[1
]}

Nordenskjold, M ^{[1
]}

Weber, G ^{[1
]}

机构：

[1] KAROLINSKA HOSP,DEPT MOLEC MED,ENDOCRINE TUMOUR UNIT,S-17176 STOCKHOLM,SWEDEN

来源：

GENOMICS | 1996年 / 31卷 / 03期

关键词：

D O I：

10.1006/geno.1996.0063

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We describe the isolation, characterization, and genomic structure of a gene, Phospholipase C beta 3 Neighboring gene (PNG), located on chromosome 11q13. The cDNA was isolated using a cosmid that also contains the phospholipase C beta 3 gene (PLCB3). PNG does not have any marked similarity to other known genes on the DNA level. However, analysis of hybridization to a panel of somatic cell hybrids indicates the existence of related sequences on chromosomes 2, 4, 7, and 22. PNG showed expression of a l-kb message in multiple tissues. The predicted protein is 199 amino acids. The gene spans approximately 2.5 kb, divided into four exons and three introns. It is located 4.4 kb upstream of PLCB3, with the 5' ends facing each other. The intergenic region has been completely sequenced, revealing separate CpG islands at both ends of this region. The islands are separated by a stretch of 2 kb, characterized by periodic alteration of the GC content. The 5'-flanking region of PNG does not contain TATA or CCAAT, suggesting a housekeeping promoter structure. (C) 1996 Academic Press, Inc.

引用

页码：380 / 384

页数：5

共 22 条

[1] MAPPING OF A NOVEL GENE FOR FAMILIAL HYPERTROPHIC CARDIOMYOPATHY TO CHROMOSOME-11
CARRIER, L
HENGSTENBERG, C
BECKMANN, JS
GUICHENEY, P
DUFOUR, C
BERCOVICI, J
DAUSSE, E
BEREBBIBERTRAND, I
WISNEWSKY, C
PULVENIS, D
FETLER, L
VIGNAL, A
WEISSENBACH, J
HILLAIRE, D
FEINGOLD, J
BOUHOUR, JB
HAGEGE, A
DESNOS, M
ISNARD, R
DUBOURG, O
KOMAJDA, M
SCHWARTZ, K
[J]. NATURE GENETICS, 1993, 4 (03) : 311 - 313
[2] EMPIRICAL PREDICTIONS OF PROTEIN CONFORMATION
CHOU, PY
FASMAN, GD
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1978, 47 : 251 - 276
[3] COMPILATION OF VERTEBRATE-ENCODED TRANSCRIPTION FACTORS
FAISST, S
MEYER, S
[J]. NUCLEIC ACIDS RESEARCH, 1992, 20 (01) : 3 - 26
[4] FUJIMORI M, 1992, AM J HUM GENET, V50, P399
[5] GRIMMOND S, 1995, HUM GENET, V95, P455
[6] HOFMANN W, 1993, ONCOGENE, V8, P3123
[7] PROMOTER-SPECIFIC ACTIVATION OF RNA POLYMERASE-II TRANSCRIPTION BY SP1
KADONAGA, JT
JONES, KA
TJIAN, R
[J]. TRENDS IN BIOCHEMICAL SCIENCES, 1986, 11 (01) : 20 - 23
[8] GENOMIC ORGANIZATION AND COMPLETE CDNA SEQUENCE OF THE HUMAN PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE-C BETA-3 GENE (PLCB3)
LAGERCRANTZ, J
CARSON, E
PHELAN, C
GRIMMOND, S
ROSEN, A
DARE, E
NORDENSKJOLD, M
HAYWARD, NK
LARSSON, C
WEBER, G
[J]. GENOMICS, 1995, 26 (03) : 467 - 472
[9] MULTIPLE ENDOCRINE NEOPLASIA TYPE-1 GENE MAPS TO CHROMOSOME-11 AND IS LOST IN INSULINOMA
LARSSON, C
SKOGSEID, B
OBERG, K
NAKAMURA, Y
NORDENSKJOLD, M
[J]. NATURE, 1988, 332 (6159) : 85 - 87
[10] LARSSON C, 1992, HUM GENET, V89, P187

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