Kinetics and modelling of an alcoholysis reaction catalyzed by cutinase immobilized on NaY zeolite

被引：17

作者：

Serralha, FN ^{[1
]}

Lopes, JM ^{[1
]}

Lemos, F ^{[1
]}

Prazeres, DMF ^{[1
]}

Aires-Barros, MR ^{[1
]}

Cabral, JMS ^{[1
]}

Ribeiro, FR ^{[1
]}

机构：

[1] Univ Tecn Lisboa, Ctr Engn Biol & Quim, Inst Super Tecn, P-1049001 Lisbon, Portugal

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2001年 / 11卷 / 4-6期

关键词：

enzymatic catalysis; NaY zeolite; cutinase; enzyme immobilization; alcoholysis;

D O I：

10.1016/S1381-1177(00)00050-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fusarium solani pisi recombinant cutinase was immobilized by adsorption on NaY zeolite. The kinetics of the alcoholysis reaction of butyl acetate with hexanol in isooctane catalyzed by cutinase immobilized on NaY zeolite, was studied. The reaction kinetics is suggested to follow a Ping-Pong bi-bi mechanism in which competitive inhibition by excess of alcohols has been identified. No evidence of any significant external diffusional limitation has been detected. The time validation of the model was successfully achieved simultaneously for 15 experimental product evolutions in a batch stirred tank reactor (BSTR) for different initial reactant concentrations. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：713 / 718

页数：6

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