Cytopathologic Detection of Circulating Tumor Cells Using the Isolation by Size of Epithelial Tumor Cell Method Promises and Pitfalls

被引：107

作者：

Hofman, Veronique J. ^{[2
,3
,4
]}

Ilie, Marius I. ^{[2
,3
,4
]}

Bonnetaud, Christelle ^{[3
]}

Selva, Eric ^{[3
]}

Long, Elodie ^{[4
]}

Molina, Thierry ^{[5
]}

Vignaud, Jean Michel ^{[6
]}

Flejou, Jean Francois ^{[7
]}

Lantuejoul, Sylvie ^{[8
]}

Piaton, Eric ^{[9
,10
]}

Butori, Catherine ^{[2
,3
,4
]}

Mourad, Nathalie ^{[11
]}

Poudenx, Michel ^{[12
]}

Bahadoran, Philippe ^{[13
]}

Sibon, Stephanie ^{[14
]}

Guevara, Nicolas ^{[15
]}

Santini, Jose ^{[15
]}

Venissac, Nicolas ^{[14
]}

Mouroux, Jerome ^{[14
]}

Vielh, Philippe ^{[16
,17
]}

Hofman, Paul M. ^{[1
,2
,3
,4
]}

机构：

[1] INSERM, ERI 21, EA 4319, Fac Med, F-06017 Nice, France

[2] Univ Nice Sophia Antipolis, Nice, France

[3] Pasteur Hosp, Human Biobank, Nice, France

[4] Pasteur Hosp, Lab Clin & Expt Pathol, Nice, France

[5] Univ Paris 05, Hotel Dieu, AP HP, Dept Pathol, Paris, France

[6] Dept Pathol, Nancy, France

[7] Hop St Antoine, Dept Pathol, F-75571 Paris, France

[8] La Tronche Hosp, Dept Pathol, Grenoble, France

[9] Sud Hosp, Dept Pathol, Lyon, France

[10] Dept Pathol, Bron, France

[11] Univ Quebec, Ctr Hosp, Dept Pathol, Quebec City, PQ, Canada

[12] Pasteur Hosp, Dept Pneumol, Nice, France

[13] Archet Il Hosp, Dept Dermatol, Nice, France

[14] Pasteur Hosp, Dept Thorac Surg, Nice, France

[15] Pasteur Hosp, Dept Otorhinolaryngol, Nice, France

[16] Inst Gustave Roussy, Dept Biopathol, Villejuif, France

[17] Inst Gustave Roussy, Lab Translat Res, Villejuif, France

来源：

AMERICAN JOURNAL OF CLINICAL PATHOLOGY | 2011年 / 135卷 / 01期

关键词：

Circulating tumor cells; Cytopathology; Diagnosis; Pitfalls; POLYMERASE-CHAIN-REACTION; PERIPHERAL-BLOOD; MESSENGER-RNA; LUNG-CANCER; RT-PCR; MOLECULAR CHARACTERIZATION; BREAST-CANCER; TIME; CARCINOMAS; PREDICTOR;

D O I：

10.1309/AJCP9X8OZBEIQVVI

中图分类号：

R36 [病理学];

学科分类号：

100103 [病原生物学];

摘要：

Detection of circulating tumor cells (CTCs) morphologically may be a promising new approach in clinical oncology. We tested the reliability of a cytomorphologic approach to identify CTCs: 808 blood samples from patients with benign and malignant diseases and healthy volunteers were examined using the isolation by size of epithelial tumor cell (ISET) method. Cells having nonhematologic features (so-called circulating nonhematologic cells [CNHCs]) were classified into 3 categories: CNHCs with malignant features, CNHCs with uncertain malignant features, and CNHCs with benign features. CNHCs were found in 11.1% and 48.9% of patients with nonmalignant and malignant pathologies, respectively (P < .001). CNHCs with malignant features were observed in 5.3% and in 43.1% of patients with nonmalignant and malignant pathologies, respectively. Cytopathologic identification of CTCs using the ISET method represents a promising field for cytopathologists. The possibility of false-positive diagnosis stresses the need for using ancillary methods to improve this approach.

引用

页码：146 / 156

页数：11

共 30 条

[1]

Tumor cells circulate in the peripheral blood of all major carcinomas but not in healthy subjects or patients with nonmalignant diseases [J].