DNA supercoiling and transcription in Escherichia coli:: The FIS connection

被引:62
作者
Travers, A
Schneider, R
Muskhelishvili, G
机构
[1] MRC, Mol Biol Lab, Cambridge CB2 2QH, England
[2] Univ Munich, Inst Genet & Mikrobiol, D-80638 Munich, Germany
关键词
FIS; DNA supercoiling; DNA microloops; transcription initiation;
D O I
10.1016/S0300-9084(00)01217-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The nucleoid-associated protein FIS modulates the topology of DNA in a growth-phase dependent manner functioning homeostatically to counteract excessive levels of negative superhelicity. We propose that this is achieved by at least two mechanisms: the physical constraint of low levels of negative superhelicity by FIS binding to DNA and by a reduction in the expression and effectiveness of DNA gyrase. In addition, high levels of expression of the fis gene do themselves require a high negative superhelical density. On DNA substrates containing phased high affinity binding sites, as exemplified by the upstream activating sequence of the tyrT promoter, FIS forms tightly bent DNA structures, or microloops, that are necessary for the optimal expression of the promoter. We suggest that these microloops compensate in part for the FIS-induced lowering of the superhelical density. (C) 2001 Societe francaise de biochimie et biologie moleculaire / Editions scientifiques ct medicales Elsevier SAS.
引用
收藏
页码:213 / 217
页数:5
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