Biochemical and morphological changes in carp (Cyprinus carpio L.) liver following exposure to copper sulfate and tannic acid

被引:125
作者
Varanka, Z
Rojik, I
Varanka, I
Nemcsók, J
Abrahám, M
机构
[1] Univ Szeged, Dept Biochem, H-6701 Szeged, Hungary
[2] Univ Szeged, Dept Comparat Physiol, H-6701 Szeged, Hungary
[3] Hungarian Acad Sci, Balaton Limnol Res Inst, H-8237 Tihany, Hungary
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY | 2001年 / 128卷 / 03期
关键词
aminotransferases; antioxidant enzymes; aquatic humics; copper sulfate; Cyprinus carpio L; electron microscopy; enzyme inhibition; liver; tannic acid;
D O I
10.1016/S1532-0456(01)00166-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
As a consequence of human activity various toxicants reach the aquatic ecosystems; humics may interact with them and may change their toxicity. Many fish are exposed to a considerable concentration of humics and pollutants. Because of paucity of data on the biochemical action of tannins in the presence of the fungicide CuSO(4) a comparative study was undertaken. The alterations of redox-parameters in carp liver were monitored and tissue necrosis was followed by measuring the plasma transaminase activities and by electron microscopy. Tannic acid, a representative phenolic/humic compound, exerted prooxidant effects in carp, which may be partially due to formation of prooxidant intermediates/end-products via its biotransformation. Alternatively, tannic acid may partially inhibit the antioxidant enzymes of fish. The response to CuSO(4) was more severe. Although tannic acid alone acted as a prooxidant in fish, electron micrographs demonstrated that it reduced the necrotizing effect of copper, which may be due to the complexing activity of tannic acid with the biomolecules of the hepatocytes and to the H(2)O(2)-degrading activity of tannin-CuSO(4) combination. Our results indicate that the heavy metal-detoxifying capacity of tannin may be significant; however, tannin-exposure alone or combined with metals may be toxic for fish due to enzyme inhibition and oxidative stress induction. (C) 2001 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:467 / 478
页数:12
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