A microfluidic array for large-scale ordering and orientation of embryos

被引：106

作者：

Chung, Kwanghun ^{[1
,2
]}

Kim, Yoosik ^{[3
,4
]}

Kanodia, Jitendra S. ^{[3
,4
]}

Gong, Emily ^{[1
,2
]}

Shvartsman, Stanislav Y. ^{[3
,4
]}

Lu, Hang ^{[1
,2
]}

机构：

[1] Georgia Inst Technol, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA

[2] Georgia Inst Technol, Parker H Petit Inst Bioengn & Biosci, Atlanta, GA 30332 USA

[3] Princeton Univ, Dept Chem & Biol Engn, Princeton, NJ 08544 USA

[4] Princeton Univ, Lewis Sigler Inst Integrat Genom, Princeton, NJ 08544 USA

来源：

NATURE METHODS | 2011年 / 8卷 / 02期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

DROSOPHILA-EMBRYO; DORSOVENTRAL PATTERN; DORSAL MORPHOGEN; GRADIENT; NUCLEAR; GENE; EXPRESSION; PROTEIN;

D O I：

10.1038/NMETH.1548

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Quantitative studies of embryogenesis require the ability to monitor pattern formation and morphogenesis in large numbers of embryos, at multiple time points and in diverse genetic backgrounds. We describe a simple approach that greatly facilitates these tasks for Drosophila melanogaster embryos, one of the most advanced models of developmental genetics. Based on passive hydrodynamics, we developed a microfluidic embryo-trap array that can be used to rapidly order and vertically orient hundreds of embryos. We describe the physical principles of the design and used this platform to quantitatively analyze multiple morphogen gradients in the dorsoventral patterning system. Our approach can also be used for live imaging and, with slight modifications, could be adapted for studies of pattern formation and morphogenesis in other model organisms.

引用

页码：171 / U103

页数：7

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[1] A MAPK docking site is critical for downregulation of Capicua by Torso and EGFR RTK signaling [J].