A modified Girard derivatizing reagent for universal profiling and trace analysis of aldehydes and ketones by electrospray ionization tandem mass spectrometry

被引：30

作者：

Johnson, David W. ^{[1
]}

机构：

[1] Womens & Childrens Hosp, Dept Med Genet, Adelaide, SA 5006, Australia

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2007年 / 21卷 / 18期

关键词：

D O I：

10.1002/rcm.3175

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

4-Hydrazino-N,N,N-trimethyl-4-oxobutanaminium iodide (HTMOB) is a modified Girard derivatizing reagent synthesized to improve the sensitivity of analysis of aldehydes and ketones with electrospray ionization tandem mass spectrometry. Compared with Girard T reagent the measured signal intensity increase is between 3.3 times (succinylacetone) and 7.0 times (17-hydroxyprogesterone). HTMOB is a universal profiling reagent for aldehydes and ketones. A neutral loss of 59 Da scan detects all aldehydes and ketones from acetone to corticosteroids. Applications described include the profiling of ketones, ketoacids and ketodiacids in the urine of children with ketosis and the profiling of long-chain aldehydes incorporated in plasma plasmalogens. Copyright (c) 2007 John Wiley & Sons, Ltd.

引用

页码：2926 / 2932

页数：7

共 12 条

[1] Heterogemini surfactants [J].

Alami, EO ;

Holmberg, K .

ADVANCES IN COLLOID AND INTERFACE SCIENCE, 2003, 100 :13-46

[2] Plasmalogen levels in serum from patients with impaired carbohydrate or lipid metabolism and in elderly subjects with normal metabolic values [J].

Brosche, T .

ARCHIVES OF GERONTOLOGY AND GERIATRICS, 2001, 32 (03) :283-294

[3] Derivatisation for the characterisation of neutral oxosteroids by electrospray and matrix-assisted laser desorption/ionisation tandem mass spectrometry:: the Girard P derivative [J].

Griffiths, WJ ;

Liu, S ;

Alvelius, G ;

Sjövall, J .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2003, 17 (09) :924-935

[4] ISATIN (INDOLE-2,3-DIONE) IN URINE AND TISSUES - DETECTION AND DETERMINATION BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY [J].

HALKET, JM ;

WATKINS, PJ ;

PRZYBOROWSKA, A ;

GOODWIN, BL ;

CLOW, A ;

GLOVER, V ;

SANDLER, M .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1991, 562 (1-2) :279-287

[5] Cleavable double-chain surfactants with one cationic and one anionic head group that form vesicles [J].

Jaeger, DA ;

Li, B ;

Clark, T .

LANGMUIR, 1996, 12 (18) :4314-4316

[6] Analysis of succinylacetone, as a Girard T derivative, in urine and dried bloodspots by flow injection electrospray ionization tandem mass spectrometry [J].

Johnson, David W. ;

Gerace, Rosemarie ;

Ranieri, Enzo ;

Trinh, Minh-Uyen ;

Fingerhut, Ralph .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2007, 21 (01) :59-63

[7]

Johnson DW, 2000, RAPID COMMUN MASS SP, V14, P2019, DOI 10.1002/1097-0231(20001115)14:21<2019::AID-RCM121>3.0.CO

[8]

2-2

[9] Ketosteroid profiling using Girard T derivatives and electrospray ionization tandem mass spectrometry: direct plasma analysis of androstenedione, 17-hydroxyprogesterone and cortisol [J].

Johnson, DW .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2005, 19 (02) :193-200

[10] Analysis of alcohols, as dimethylglycine esters, by electrospray ionization tandem mass spectrometry [J].

Johnson, DW .

JOURNAL OF MASS SPECTROMETRY, 2001, 36 (03) :277-283

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