Population growth kinetics of the nematode, Steinernema feltiae, in submerged monoxenic culture

被引:30
作者
Chavarría-Hernández, N [1 ]
de la Torre, M [1 ]
机构
[1] Inst Politecn Nacl, CINVESTAV, Biotechnol & Bioengn Dept, Mexico City 07360, DF, Mexico
关键词
biological control; entomopathogenic nematode; monoxenic culture; population growth kinetics; Steinernema feltiae;
D O I
10.1023/A:1005694709366
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Monoxenic cultures of the nematode, Steinernema feltiae, were carried out on two complex liquid media: P1, mainly soybean flour/egg yolk/yeast extract, and P2, mainly egg yolk/yeast extract. Up to 140 000-200 000 nematodes ml(-1) were produced within 7 days, and more than 95% of the final population was in the infective juvenile stage. The total nematode concentration growth curve had a sigmoidal shape. Nematode population growth kinetics were modelled using a re-parameterised Gompertz model. Yeast extract concentration appeared to be a key factor for obtaining high nematode concentrations.
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页码:311 / 315
页数:5
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