Sigma factor genes sigC, sipE, and sigG are upregulated in heterocysts of the cyanobacterium Anabaena sp strain PCC 7120

被引：37

作者：

Aldea, M. Ramona ^{[1
]}

Mella-Herrera, Rodrigo A. ^{[1
]}

Golden, James W. ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA

来源：

JOURNAL OF BACTERIOLOGY | 2007年 / 189卷 / 22期

关键词：

D O I：

10.1128/JB.00821-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We used gfp transcriptional fusions to investigate the regulation of eight sigma factor genes during heterocyst development in the cyanobacterium Anabaena sp. strain PCC 7120. Reporter strains containing gfp fusions with the upstream regions of sigB2, sigD, sigI, and sigJ did not show developmental regulation. Time-lapse microscopy of sigC, sigE, and sigG reporter strains showed increased green fluorescent protein fluorescence in differentiating cells at 4 h, 16 h, and 9 h, respectively, after nitrogen step down.

引用

页码：8392 / 8396

页数：5

共 14 条

[1] IDENTIFICATION OF MULTIPLE RNA-POLYMERASE SIGMA FACTOR HOMOLOGS IN THE CYANOBACTERIUM ANABAENA SP STRAIN PCC 7120 - CLONING, EXPRESSION, AND INACTIVATION OF THE SIGB AND SIGC GENES [J].

BRAHAMSHA, B ;

HASELKORN, R .

JOURNAL OF BACTERIOLOGY, 1992, 174 (22) :7273-7282

[2] Heterocyst development in Anabaena [J].

Golden, JW ;

Yoon, HS .

CURRENT OPINION IN MICROBIOLOGY, 2003, 6 (06) :557-563

[3] INDEPENDENT REGULATION OF NIFHDK OPERON TRANSCRIPTION AND DNA REARRANGEMENT DURING HETEROCYST DIFFERENTIATION IN THE CYANOBACTERIUM ANABAENA SP STRAIN PCC 7120 [J].

GOLDEN, JW ;

WHORFF, LL ;

WIEST, DR .

JOURNAL OF BACTERIOLOGY, 1991, 173 (22) :7098-7105

[4] Specificity crosstalk among group 1 and group 2 sigma factors in the cyanobacterium Synechococcus sp PCC7942:: in vitro specificity and a phylogenetic analysis [J].

Goto-Seki, A ;

Shirokane, M ;

Masuda, S ;

Tanaka, K ;

Takahashi, H .

MOLECULAR MICROBIOLOGY, 1999, 34 (03) :473-484

[5] Multiple sigma subunits and the partitioning of bacterial transcription space [J].

Gruber, TM ;

Gross, CA .

ANNUAL REVIEW OF MICROBIOLOGY, 2003, 57 :441-466

[6] Growth phase-dependent activation of nitrogen-related genes by a control network of group 1 and group 2 σ factors in a cyanobacterium [J].

Imamura, S ;

Tanaka, K ;

Shirai, M ;

Asayama, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (05) :2668-2675

[7] Antagonistic dark/light-induced SigB/SigD, group 2 sigma factors, expression through redox potential and their roles in cyanobacteria [J].

Imamura, S ;

Asayama, M ;

Takahashi, H ;

Tanaka, K ;

Takahashi, H ;

Shirai, M .

FEBS LETTERS, 2003, 554 (03) :357-362

[8] Identification and inactivation of three group 2 sigma factor genes in Anabaena sp strain PCC 7120 [J].

Khudyakov, IY ;

Golden, JW .

JOURNAL OF BACTERIOLOGY, 2001, 183 (22) :6667-6675

[9] PlmA, a new member of the GntR family, has plasmid maintenance functions in Anabaena sp strain PCC 7120 [J].

Lee, MH ;

Scherer, M ;

Rigali, S ;

Golden, JW .

JOURNAL OF BACTERIOLOGY, 2003, 185 (15) :4315-4325

[10] GENERIC ASSIGNMENTS, STRAIN HISTORIES AND PROPERTIES OF PURE CULTURES OF CYANOBACTERIA [J].

RIPPKA, R ;

DERUELLES, J ;

WATERBURY, JB ;

HERDMAN, M ;

STANIER, RY .

JOURNAL OF GENERAL MICROBIOLOGY, 1979, 111 (MAR) :1-61

← 1 2 →