Recognition of B-cell epitopes of the 65 kDa HSP in Behcet's disease

被引:58
作者
Direskeneli, H
Hasan, A
Shinnick, T
Mizushima, Y
VanderZee, R
Fortune, F
Stanford, MR
Lehner, T
机构
[1] CTR DIS CONTROL,HANSENS DIS LAB,DIV BACTERIAL DIS,ATLANTA,GA 30333
[2] ST MARIANNA UNIV,SCH MED,DEPT INTERNAL MED,KAWASAKI,KANAGAWA,JAPAN
[3] INST INFECT DIS IMMUNOL,UTRECHT,NETHERLANDS
[4] UMDS,GUYS HOSP,DEPT IMMUNOL,LONDON SE1 9RT,ENGLAND
关键词
D O I
10.1046/j.1365-3083.1996.d01-53.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
B-cell epitopes of the mycobacterial 65 kDa heat shock protein (HSP) were mapped in sera from patients with Behcet's (BD). A series of 47 overlapping synthetic peptides (15(ers)) derived from the sequence of the Mycobacterium tuberculosis 65 kDa HSP was used in ELISA. Significant increases in IgA and IgG antibody levels were observed with peptides 111-125, 154-172 and 311-326 in sera from BD, compared with those from controls. Homologous peptides derived from the sequence of the human mitochondrial 60 kDa HSP were then examined. Peptides 136-150 and 336-351 showed comparable results to the homologous mycobacterial peptides 111-125 and 311-326, respectively. The B-cell epitopes defined in this investigation overlap with the T-cell epitopes the authors have previously reported in BD. Inhibition studies are consistent with the view that antibodies to each of the three B-cell epitope peptides represent a small proportion of the total B-cell epitope repertoire elicited by the 65 or 60 kD HSP. Sequential antibody studies suggest that IgA and IgG antibody titres to one or all three peptides tested map increase during exacerbations of ocular disease. The functional role of these antibodies needs to be determined, but the peptides may be involved in the immunopathogenesis of BD as they can induce experimental uveitis in Lewis rats, which is a principal manifestation of BD.
引用
收藏
页码:464 / 471
页数:8
相关论文
共 43 条
  • [1] BURTONKEE EJ, 1981, J LAB CLIN MED, V97, P559
  • [2] AUTOIMMUNITY, MICROBIAL IMMUNITY AND THE IMMUNOLOGICAL HOMUNCULUS
    COHEN, IR
    YOUNG, DB
    [J]. IMMUNOLOGY TODAY, 1991, 12 (04): : 105 - 110
  • [3] AUTOIMMUNITY TO CHAPERONINS IN THE PATHOGENESIS OF ARTHRITIS AND DIABETES
    COHEN, IR
    [J]. ANNUAL REVIEW OF IMMUNOLOGY, 1991, 9 : 567 - 589
  • [4] JUVENILE RHEUMATOID-ARTHRITIS PATIENTS MANIFEST IMMUNE REACTIVITY TO THE MYCOBACTERIAL 65-KDA HEAT-SHOCK PROTEIN, TO ITS 180-188-PEPTIDE, AND TO A PARTIALLY HOMOLOGOUS PEPTIDE OF THE PROTEOGLYCAN LINK PROTEIN
    DANIELI, MG
    MARKOVITS, D
    GABRIELLI, A
    CORVETTA, A
    GIORGI, PL
    VANDERZEE, R
    VANEMBDEN, JDA
    DANIELI, G
    COHEN, IR
    [J]. CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY, 1992, 64 (02): : 121 - 128
  • [5] ANTIBODIES TO MYCOBACTERIAL 65 KDA HEAT-SHOCK PROTEIN IN SYSTEMIC-SCLEROSIS (SCLERODERMA)
    DANIELI, MG
    CANDELA, M
    RICCIATTI, AM
    REGINELLI, R
    DANIELI, G
    COHEN, IR
    GABRIELLI, A
    [J]. JOURNAL OF AUTOIMMUNITY, 1992, 5 (04) : 443 - 452
  • [6] EGLIN RP, 1982, LANCET, V2, P1356
  • [7] VACCINATION AGAINST AUTOIMMUNE MOUSE DIABETES WITH A T-CELL EPITOPE OF THE HUMAN 65-KDA HEAT-SHOCK PROTEIN
    ELIAS, D
    RESHEF, T
    BIRK, OS
    VANDERZEE, R
    WALKER, MD
    COHEN, IR
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (08) : 3088 - 3091
  • [8] FORTUNE F, 1990, CLIN EXP IMMUNOL, V82, P326
  • [9] FRIEDLAND JS, 1993, CLIN EXP IMMUNOL, V91, P58
  • [10] RECOGNITION OF A MYCOBACTERIA-SPECIFIC EPITOPE IN THE 65-KD HEAT-SHOCK PROTEIN BY SYNOVIAL FLUID-DERIVED T-CELL CLONES
    GASTON, JSH
    LIFE, PF
    JENNER, PJ
    COLSTON, MJ
    BACON, PA
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1990, 171 (03) : 831 - 841