Single step isolation of sildenafil from commercially available Viagra™ tablets

被引：22

作者：

Francis, SH ^{[1
]}

Sekhar, KR ^{[1
]}

Rouse, AB ^{[1
]}

Grimes, KA ^{[1
]}

Corbin, JD ^{[1
]}

机构：

[1] Vanderbilt Univ, Sch Med, Dept Physiol & Mol Biophys, Nashville, TN 37232 USA

来源：

INTERNATIONAL JOURNAL OF IMPOTENCE RESEARCH | 2003年 / 15卷 / 05期

关键词：

sildenafil; Viagra (TM); phosphodiesterase-5; PDE5; inhibitors; cGMP;

D O I：

10.1038/sj.ijir.3901040

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

Sildenafil, the active ingredient in Viagra(TM), ahs been purified from commercially available tablets. The purification, using Sephadex G25 chromatography under conditions of low ionic strength, is simple and inexpensive. Sildenafil purified according to this protocol has been characterized with respect to its IC50 for PDE5, its ultraviolet absorption profile, and by collision-induced dissociation fingerprinting, positive ion nanospray, and MALDI mass spectrometry. Tritated sildenafil (6 Ci/mmol) was prepared commercially using the sildenafil purified by this protocol and was verified to retain the potency of unlabeled sildenafil. This protocol and similar procedures will allow investigators to easily isolate sufficient amounts of sildenafil or other PDE5 inhibitors for conducting biochemical and in vitro studies of drug action.

引用

页码：369 / 372

页数：4

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