Plant regeneration from callus in a range of Phaseolus acutifolius A. Gray genotypes

被引:28
作者
Dillen, W [1 ]
DeClercq, J [1 ]
VanMontagu, M [1 ]
Angenon, G [1 ]
机构
[1] STATE UNIV GHENT VIB, DEPT GENET, GENET LAB, B-9000 GHENT, BELGIUM
关键词
Fabaceae; legumes; organogenesis; phaseolus; tepary bean; tissue culture;
D O I
10.1016/0168-9452(96)04419-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The ability of a range of Phaseolus acutifolius A. Gray genotypes to regenerate shoots from callus was investigated. Shoots and viable plants were obtained from green nodular callus derived from buds and pedicels. Seven of 12 genotypes tested produced morphologically normal shoots. Regenerants of six genotypes were established in the greenhouse. The regeneration response reflected the taxonomic subdivision of the species in that P. acutifolius var. acutifolius was responsive whereas var. tenuifolius was not. The 'regeneration competence' trait was present in both the wild and the domesticated gene pools. The possible application of this trait for the genetic improvement of the related and economically more important species P. vulgaris is discussed.
引用
收藏
页码:81 / 88
页数:8
相关论文
共 37 条