Performances of a capillary electrophoresis method for the determination of meso-inositol in a tablet formulation

A capillary zone electrophoresis method is reported for the determination of meso-inositol in a tablet formulation. Separation was carried out in a fused-silica capillary 50.2 cm long (40.2 cm effective length) x 50 mum internal diameter at a potential of 15 kV and 60 degreesC using a 50 mM tetraborate buffer. Detection was by W absorbance at 195 nm. An internal standard (D-fructose) was used to increase method precision. Aqueous standard and test solutions of tablets (4 g l(-1)) were hydrodynamically injected at a pressure of 0.0041 MPa for 6 s. Validation tests demonstrated that the method was reliable and rugged for routine use in quality control. Repeatability of relative corrected peak areas meso-inositol/D-fructose was approximately 2% for 10 injections and 10 preparations of test solutions, respectively Inter-day (10 days) and inter-capillary (two different suppliers) precision was 1.7% for the assay. No influence of day or capillary on the assay was found. Advantages of CE for the purpose of this analysis was the short total analysis time (less than 7 min), the rapid set-up of the instrumentation and the low running cost. (C) 2001 Elsevier Science B.V. All rights reserved.