Increased concentrations of the oxidative DNA adduct 7,8-dihydro-8-oxo-2-deoxyguanosine in the germ-line of men with type 1 diabetes

被引：54

作者：

Agbaje, I. M. ^{[1
]}

McVicar, C. M. ^{[1
]}

Schock, B. C. ^{[2
]}

McClure, N. ^{[2
]}

Atkinson, A. B. ^{[3
]}

Rogers, D. ^{[1
]}

Lewis, S. E. M. ^{[1
]}

机构：

[1] Queens Univ Belfast, Ctr Clin & Populat Sci, Inst Clin Sci, Reprod Med Res Grp, Belfast BT12 6BJ, Antrim, North Ireland

[2] Queens Univ Belfast, Inst Clin Sci, Div Med & therapeut, Belfast BT12 6BJ, Antrim, North Ireland

[3] Royal Victoria Hosp, Reg Ctr Endicrinol & Diabet, Belfast BT12 6BA, Antrim, North Ireland

来源：

REPRODUCTIVE BIOMEDICINE ONLINE | 2008年 / 16卷 / 03期

关键词：

diabetes mellitus; human spermatozoa; 8-hydroxydeoxyguanosine; nuclear DNA; oxidative stress;

D O I：

10.1016/S1472-6483(10)60602-5

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The effects of diabetes mellitus on male reproductive health have not been clearly defined. A previous publication from this group reported significantly higher levels of nuclear DNA fragmentation and mitochondrial DNA deletions in spermatozoa from men with type 1 diabetes. This study compared semen profiles, sperm DNA fragmentation and levels of oxidative DNA modification in spermatozoa of diabetic and non-diabetic men. Semen samples from 12 non-diabetic, fertile men and 11 type 1 diabetics were obtained and subjected to conventional light microscopic semen analysis. Nuclear DNA fragmentation was assessed using an alkaline Comet assay and concentrations of 7,8-dihydro-8-oxo-2-deoxyguanosine (8-OHdG), an oxidative adduct of the purine guanosine, were assessed by high-performance liquid chromatography. Conventional semen profiles were similar in both groups, whilst spermatozoa from type I diabetics showed significantly higher levels of DNA fragmentation (44% versus 27%; P < 0.05) and concentrations of 8-OHdG (3.6 versus 2.0 molecules of 8-OHdG per 101 molecules of deoxyguanosine; P < 0.05). Furthermore, a positive correlation was observed between DNA fragmentation and concentrations of 8-OHdG per 10(5) molecules of deoxyguanosine, (r(s) = 0.7, P < 0.05). The genomic damage evident in spermatozoa of type I diabetics may have important implications for their fertility and the outcome of pregnancies fathered by these individuals.

引用

页码：401 / 409

页数：9

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