Fine structure in cellulose microfibrils:: NMR evidence from onion and quince

被引:100
作者
Ha, MA
Apperley, DC
Evans, BW
Huxham, M
Jardine, WG
Viëtor, RJ
Reis, D
Vian, B
Jarvis, MC [1 ]
机构
[1] Univ Glasgow, Dept Chem, Glasgow G12 8QQ, Lanark, Scotland
[2] Univ Durham, EPSRC, Solid State NMR Serv, Durham DH1 3LE, England
[3] INRA, Lab Pathol Vegetale, F-75231 Paris, France
关键词
D O I
10.1046/j.1365-313x.1998.00291.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
It has been controversial for many years whether in the cellulose of higher plants, the microfibrils are aggregates of 'elementary fibrils', which have been suggested to be about 3.5 nm in diameter. Solid-state NMR spectroscopy was used to examine two celluloses whose fibril diameters had been established by electron microscopy: onion (8-10 nm, but containing 40% of xyloglucan as well as cellulose) and quince (2 nm cellulose core). Both of these forms of cellulose contained crystalline units of similar size, as estimated from the ratio of surface to interior chains, and the time required for proton magnetisation to diffuse from the surface to the interior. It is suggested that the onion microfibrils must therefore be constructed from a number of cellulose subunits 2 nm in diameter, smaller than the 'elementary fibrils' envisaged previously The size of these subunits would permit a hexagonal arrangement resembling the cellulose synthase complex.
引用
收藏
页码:183 / 190
页数:8
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