Gadd45β mediates the protective effects of CD40 costimulation against Fas-induced apoptosis

In B lymphocytes, induction of apoptosis or programmed cell death (PCD) by Fas (CD95/APO-1) is suppressed by the triggering of CD40. This suppression controls various aspects of the humoral immune response, including antibody affinity maturation. The opposing effects of these receptors are also crucial to B-cell homeostasis, autoimmune disease, and cancer. Cytoprotection by CD40 involves activation of protective genes mediated by NF-kappaB transcription factors; however, its basis remains poorly understood. Here, we report that, in B cells, Gadd45beta is induced by CD40 through a mechanism that requires NF-kappaB and that this induction suppresses Fas-mediated killing. Importantly, up-regulation of Gadd45beta by CD40 precedes Fas-induced caspase activation, as well as up-regulation of other NF-kappaB-controlled inhibitors of apoptosis such as Bcl-x(L) and c-FLIPL. In the presence of Gadd45beta, the Fas-induced apoptotic cascade is halted at mitochondria. However, in contrast to Bcl-x(L), Gadd45beta is unable to hamper the "intrinsic" pathway for apoptosis and in fact appears to block Fas cytotoxicity herein by suppressing a mitochondria-targeting mechanism activated by this receptor. These findings identify Gadd45beta as a critical mediator of the prosurvival response to CD40 stimulation and provide important new insights into the apoptotic mechanism that is triggered by Fas in B cells. (C) 2003 by The American Society of Hematology.