Simultaneous measurement of monoamines, their metabolites and 2,3- and 2,5-dihydroxybenzoates by high-performance liquid chromatography with electrochemical detection. Application to rat brain dialysates

被引：18

作者：

Duine, JM ^{[1
]}

Floch, F ^{[1
]}

Cann-Moisan, C ^{[1
]}

Mialon, P ^{[1
]}

Caroff, J ^{[1
]}

机构：

[1] Fac Med, Physiol Lab, F-29285 Brest, France

来源：

JOURNAL OF CHROMATOGRAPHY B | 1998年 / 716卷 / 1-2期

关键词：

monoamines; dihydroxybenzoates;

D O I：

10.1016/S0378-4347(98)00301-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reversed-phase chromatographic method with electrochemical detection was developed for the simultaneous determination of 2,3- and 2,5-dihydroxybenzoates, indicators of in vivo hydroxyl free radical formation, monoamines (NE, DA, 5-HT) and their metabolites (MHPG, DOPAC, HVA, 3MT, 5-HIAA). Linearity was observed from 10 pg to 10 ng injected. Reproducibility is correct (C.V. about 9%) except for 3MT and 5-HT. The limit of detection for almost all products was about 20 pg injected on the column. An application of this method in the study of the neurotoxicity of high pressure oxygen in rat is described. The Limit of quantification for all compounds was 5 ng/ml except for HVA (10 ng/ml). Some basal levels DA, 5-HT, 5-HIAA, HVA, DOPAC, 3MT, 2,5-DHBA and 2,3-DHBA in microdialysates coming from striatum of normoxic restrained rats are given. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：350 / 353

页数：4

共 13 条

[1]

CANNMOISAN C, 1996, J LIQ CHROMATOGR R T, V19, P927

[2]

CHEN NH, 1995, J NEUROCHEM, V64, P1585

[3] EFFECTS OF TRANSIENT FOREBRAIN ISCHEMIA AND PARGYLINE ON EXTRACELLULAR CONCENTRATIONS OF DOPAMINE, SEROTONIN, AND THEIR METABOLITES IN THE RAT STRIATUM AS DETERMINED BY INVIVO MICRODIALYSIS [J].