Kinetics of cholesterol extraction from lipid membranes by methyl-β-cyclodextrin -: A surface plasmon resonance approach

被引：64

作者：

Besenicar, Mojca Podlesnik ^{[1
]}

Bavdek, Andrej ^{[1
]}

Kladnik, Ales ^{[1
]}

Macek, Peter ^{[1
]}

Anderluh, Gregor ^{[1
]}

机构：

[1] Univ Ljubljana, Dept Biol, Fac Biotecn, Ljubljana 1000, Slovenia

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2008年 / 1778卷 / 01期

关键词：

lipid membrane; cholesterol extraction; cyclodextrin; surface plasmon resonance; biacore;

D O I：

10.1016/j.bbamem.2007.09.022

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The kinetics of cholesterol extraction from cellular membranes is complex and not yet completely understood. In this paper we have developed an experimental approach to directly monitor the extraction of cholesterol from lipid membranes by using surface plasmon resonance and model lipid systems. Methyl-beta-cyclodextrin was used to selectively remove cholesterol from large unilamellar vesicles of various compositions. The amount of extracted cholesterol is highly dependent on the composition of lipid membrane, i.e. the presence of sphingomyelin drastically reduced and slowed down cholesterol extraction by methyl-beta-cyclodextrin. This was confirmed also in the erythrocyte ghosts system, where more cholesterol was extracted after erythrocytes were treated with sphingomyelinase. We further show that the kinetics of the extraction is mono-exponential for mixtures of 1,2-dioleoyl-sn-glycero-3-phosphocholine and cholesterol. The kinetics is complex for ternary lipid mixtures composed of 1,2-dioleoyl-sn-glycero-3-phosphocho line, bovine brain sphingomyelin and cholesterol. Our results indicate that the complex kinetics observed in experiments with cells may be the consequence of lateral segregation of lipids in cell plasma membrane. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：175 / 184

页数：10

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