RNAi-mediated silencing of leptin gene expression increases cell death in C6 glioblastoma cells

被引：15

作者：

Brown, R

Morash, B

Ur, E

Wilkinson, M

机构：

[1] Dalhousie Univ, Fac Med, Dept Obstet & Gynaecol, IWK Hlth Ctr, Halifax, NS B3K 6R8, Canada

[2] Dalhousie Univ, Fac Med, Dept Physiol & Biophys, Halifax, NS B3K 6R8, Canada

[3] Dalhousie Univ, Fac Med, Div Endocrinol & Metab, Halifax, NS B3K 6R8, Canada

来源：

MOLECULAR BRAIN RESEARCH | 2005年 / 139卷 / 02期

关键词：

leptin; brain; reverse transcription polymerase chain reaction; small interfering RNA; RNA interference; cell death;

D O I：

10.1016/j.molbrainres.2005.05.009

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We previously demonstrated that the brain, pituitary, and C6 glioblastoma cells express leptin. To determine the physiological role of brain-derived leptin, we sought to selectively silence its expression using RNA interference (RNAi) in vitro. One of four potential targets, siRNA L7, reduced leptin mRNA by 50% (P < 0.05) and protein by 55% (P < 0.0001) in C6 cells. RNAi also induced a twofold increase in cell death as seen by ethidium homodimer-1 (P < 0.015) and TUNEL (P < 0.005) staining. These data suggest that endogenous leptin may be a critical factor promoting cell survival in the brain. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：357 / 360

页数：4

共 28 条

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