Measurement of green fluorescence protein in plants: quantification, correlation to expression, rapid screening and differential gene expression

被引：22

作者：

Blumenthal, A ^{[1
]}

Kuznetzova, L ^{[1
]}

Edelbaum, O ^{[1
]}

Raskin, V ^{[1
]}

Levy, M ^{[1
]}

Sela, I ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Otto Warburg Ctr Biotechnol, Fac Agr, IL-76100 Rehovot, Israel

来源：

PLANT SCIENCE | 1999年 / 142卷 / 01期

关键词：

green fluorescence protein; plants; gene expression monitoring and quantification;

D O I：

10.1016/S0168-9452(98)00249-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A set-up for detection of green fluorescence protein (GFP), enabling rapid screening of transgenic tobacco is described. Fluorescence levels were proportional to expression levels, and expressing plants were visually selected from a transgenic population. Quantification of GFP expression indicated that the 35S CaMV promoter drives expression of this reporter gene to different levels in different tissues, corroborating previous similar observation with GUS, indicating that constitutive expression varies quantitatively in different tissues. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：93 / 99

页数：7

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