Filarial susceptibility and effects of Wolbachia in Aedes pseudoscutellaris mosquitoes

被引:16
作者
Dutton, TJ [1 ]
Sinkins, SP [1 ]
机构
[1] Univ Liverpool, Liverpool Sch Trop Med, Vector Res Grp, Liverpool L3 5QA, Merseyside, England
关键词
Aedes pseudoscutellaris; Ae; katherinensis; polynesiensis; Brugia pahangi; Wolbachia; cytoplasmic incompatibility; filariasis vectors; maternal inheritance; vector competence; Fiji; Polynesia;
D O I
10.1111/j.0269-283X.2005.00557.x
中图分类号
Q96 [昆虫学];
学科分类号
摘要
The mosquito Aedes pseudoscutellaris (Theobald), a member of the Aedes (Stegomyia) scutellaris complex (Diptera: Culicidae), is an important vector of subperiodic Wuchereria bancrofti (Cobbold) (Spirurida: Onchocercidae), causing human lymphatic filariasis, on South Pacific islands. Maternal inheritance of filarial susceptibility in the complex has previously been asserted, and larval tetracycline treatment reduced susceptibility; the maternally inherited Wolbachia in these mosquitoes were suggested to be responsible. To investigate the relationship of these two factors, we eliminated Wolbachia from a strain of Ae. pseudoscutellaris by tetracycline treatment, and tested filarial susceptibility of the adult female mosquitoes using Brugia pahangi (Edeson & Buckley). Filarial susceptibility was not significantly different in Wolbachia-free and infected lines of Ae. pseudoscutellaris, suggesting that the Wolbachia in these mosquitoes do not influence vector competence. Crosses between Wolbachia-infected males and uninfected females of Ae. pseudoscutellaris showed cytoplasmic incompatibility (CI), i.e. no eggs hatched, unaffected by larval crowding or restricted nutrient availability, whereas these factors are known to affect CI in Drosophila simulans. Reciprocal crosses between Ae. pseudoscutellaris and Ae. katherinensis Woodhill produced no progeny, even when both parents were Wolbachia-free, suggesting that nuclear factors are responsible for this interspecific sterility.
引用
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页码:60 / 65
页数:6
相关论文
共 32 条
[1]   Phylogeny of Wolbachia in filarial nematodes [J].
Bandi, C ;
Anderson, TJC ;
Genchi, C ;
Blaxter, ML .
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1998, 265 (1413) :2407-2413
[2]  
BEHBAHANI A, 2005, IN PRESS MED VET ENT, V19
[3]   A RIBOSOMAL-RNA GENE PROBE DIFFERENTIATES MEMBER SPECIES OF THE ANOPHELES-GAMBIAE COMPLEX [J].
COLLINS, FH ;
MENDEZ, MA ;
RASMUSSEN, MO ;
MEHAFFEY, PC ;
BESANSKY, NJ ;
FINNERTY, V .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1987, 37 (01) :37-41
[4]   SUSCEPTIBILITY OF APOSYMBIOTIC CULEX-QUINQUEFASCIATUS TO WUCHERERIA-BANCROFTI [J].
CURTIS, CF ;
ELLIS, DS ;
DOYLE, PE ;
HILL, N ;
RAMJI, BD ;
IRUNGU, LW ;
TOWNSON, H .
JOURNAL OF INVERTEBRATE PATHOLOGY, 1983, 41 (02) :214-223
[5]  
DEV V, 1982, EVOLUTIONARY SIGNIFI, P89
[6]   A novel technique for removing Wolbachia infections from Aedes albopictus (Diptera: Culicidae) [J].
Dobson, SL ;
Rattanadechakul, W .
JOURNAL OF MEDICAL ENTOMOLOGY, 2001, 38 (06) :844-849
[7]  
DUHRKOPF RE, 1981, MOSQ NEWS, V41, P729
[8]   Strain-specific quantification of Wolbachia density in Aedes albopictus and effects of larval rearing conditions [J].
Dutton, TJ ;
Sinkins, SP .
INSECT MOLECULAR BIOLOGY, 2004, 13 (03) :317-322
[9]   VERTICAL TRANSMISSION OF DENGUE VIRUSES BY MOSQUITOS OF THE AEDES-SCUTELLARIS GROUP [J].
FREIER, JE ;
ROSEN, L .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1987, 37 (03) :640-647
[10]  
Hoffmann AA, 1997, INFLUENTIAL PASSENGERS, P42