Factor XI/ADAMTS13 complexes are quantitatively insignificant in human plasma

被引：3

作者：

Anderson, Patricia J. ^{[1
]}

Gailani, David ^{[3
]}

Feys, Hendrik B. ^{[4
]}

Gao, Weiqiang ^{[2
]}

Majerus, Elaine M. ^{[1
]}

Vanhoorelbeke, Karen ^{[4
]}

Sadler, J. Evan ^{[1
,2
]}

机构：

[1] Washington Univ, Sch Med, Dept Med, 660 S Euclid,Box 8066, St Louis, MO 63110 USA

[2] Washington Univ, Sch Med, Howard Hughes Med Inst, St Louis, MO 63110 USA

[3] Vanderbilt Univ, Dept Pathol & Med, Nashville, TN 37232 USA

[4] Katholieke Univ Leuven, Interdisciplinary Res Ctr, Lab Thrombosis Res, B-8500 Kortrijk, Belgium

来源：

HAEMATOLOGICA | 2007年 / 92卷 / 10期

基金：

美国国家卫生研究院;

关键词：

von Willebrand Factor; factor XI; ADAMTS13;

D O I：

10.3324/haematol.11478

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Reportedly, complexes between factor XI and ADAMTS13 are detected with a commercial ADAMTS13/FXI ELISA kit in plasma and are decreased in thrombotic thrombocytopenic purpura (TTP). Using this kit, control and TTP patient plasma contained varying amounts of signal (25-670% of a reference plasma) but no signal was observed for mixtures of recombinant enzymes, suggesting little interaction. ADAMTS13/FXI complexes were undetectable by immunoprecipitation or gel filtration chromatography in control plasma or mixtures of recombinant proteins. These results suggest that ADAMTS13/FXI complexes are insignificant in plasma and unlikely to affect the function of either protein during normal hemostasis or in TTP.

引用

页码：1419 / 1422

页数：4

共 18 条

[1]

ACKERS GK, 1967, J BIOL CHEM, V242, P3237

[2] Zinc and calcium ions cooperatively modulate ADAMTS13 activity [J].

Anderson, PJ ;

Kokame, K ;

Sadler, JE .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (02) :850-857

[3]

BOUMA BN, 1977, J BIOL CHEM, V252, P6432

[4] Structural and functional correlation of ADAMTS13 [J].

Dong, Jing-Fei .

CURRENT OPINION IN HEMATOLOGY, 2007, 14 (03) :270-276

[5] ADAMTS-13 plasma level determination uncovers antigen absence in acquired thrombotic thrombocytopenic purpura and ethnic differences [J].

Feys, HB ;

Liu, F ;

Dong, N ;

Pareyn, I ;

Vauterin, S ;

Vandeputte, N ;

Noppe, W ;

Ruan, C ;

Deckmyn, H ;

Vanhoorelbeke, K .

JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2006, 4 (05) :955-962

[6] Partial amino acid sequence of purified von Willebrand factor-cleaving protease [J].

Gerritsen, HE ;

Robles, R ;

Lämmle, B ;

Furlan, M .

BLOOD, 2001, 98 (06) :1654-1661

[7] Binding of ADAMTS13 to von Willebrand factor [J].

Majerus, EM ;

Anderson, PJ ;

Sadler, JE .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (23) :21773-21778

[8] ADAMTS13 assays and ADAMTS13-deficient mice [J].

Miyata, Toshiyuki ;

Kokame, Koichi ;

Banno, Fumiaki ;

Shin, Yongchol ;

Akiyama, Masashi .

CURRENT OPINION IN HEMATOLOGY, 2007, 14 (03) :277-283

[9] A covalent oxidoreductase intermediate in propeptide-dependent von Willebrand factor multimerization [J].

Purvis, AR ;

Sadler, JE .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (48) :49982-49988

[10]

Sadler J Evan, 2006, Hematology Am Soc Hematol Educ Program, P415

← 1 2 →