Preparation of a branched DNA self-assembled monolayer toward sensitive DNA biosensors

被引：36

作者：

Nakamura, F

Ito, E

Sakao, Y

Ueno, N

Gatuna, IN

Ohuchi, FS

Hara, M

机构：

[1] RIKEN, Frontier Res Syst, Local Spatio Temporal Funct Lab, Wako, Saitama 3510198, Japan

[2] JST, PRESTO, Kawaguchi, Saitama 3320012, Japan

[3] Chiba Univ, Fac Engn, Inage Ku, Chiba 2360022, Japan

[4] Univ Washington, Dept Mat Sci & Engn, Seattle, WA 98195 USA

来源：

NANO LETTERS | 2003年 / 3卷 / 08期

关键词：

D O I：

10.1021/nl0342794

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

To realize an efficient hybridization of oligonucleotides, a DNA self-assembled monolayer (SAM) composed of branched probe DNA containing single- and double-stranded portions was prepared on a gold substrate. Hybridization using the SAM was monitored in situ by surface plasmon resonance (SPR). By controlling the surface coverage of the probe DNA, hybridization efficiency was optimized. SPR results strongly indicate that all probe sites are accessible for hybridization under optimized conditions regardless of the position. To realize an efficient hybridization of oligonucleotides, a DNA self-assembled monolayer (SAM) composed of branched probe DNA containing single- and double-stranded portions was prepared on a gold substrate. Hybridization using the SAM was monitored in situ by surface plasmon resonance (SPR). By controlling the surface coverage of the probe DNA, hybridization efficiency was optimized. SPR results strongly indicate that all probe sites are accessible for hybridization under optimized conditions regardless of the position.

引用

页码：1083 / 1086

页数：4

共 21 条

[1] ENERGY CALIBRATION IN ELECTRON-SPECTROSCOPY AND THE RE-DETERMINATION OF SOME REFERENCE ELECTRON-BINDING ENERGIES [J].