Conversion of NfsB, a minor Escherichia coli nitroreductase, to a flavin reductase similar in biochemical properties to FRase I, the major flavin reductase in Vibrio fischeri, by a single amino acid substitution

被引：43

作者：

Zenno, SH

Koike, H

Tanokura, M

Saigo, K

机构：

[1] UNIV TOKYO,BIOTECHNOL RES CTR,BUNKYO KU,TOKYO 106,JAPAN

[2] CHISSO CORP,YOKOHAMA RES CTR,KANAZAWA KU,YOKOHAMA,KANAGAWA 236,JAPAN

来源：

JOURNAL OF BACTERIOLOGY | 1996年 / 178卷 / 15期

关键词：

D O I：

10.1128/jb.178.15.4731-4733.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

NfsB is an oxygen-insensitive nitroreductase of Escherichia coli with significant amino acid sequence homology to the major flavin reductase (FRase I) of Vibrio fischeri. Here, we show that NfsB is convertible to an FRase I-like flavin reductase three times as active as the authentic FRase I by a single amino acid substitution in the least-conserved region.

引用

页码：4731 / 4733

页数：3

共 14 条

[1] THE BIOACTIVATION OF 5-(AZIRIDIN-1-YL)-2,4-DINITROBENZAMIDE (CB1954) .1. PURIFICATION AND PROPERTIES OF A NITROREDUCTASE ENZYME FROM ESCHERICHIA-COLI - A POTENTIAL ENZYME FOR ANTIBODY-DIRECTED ENZYME PRODRUG THERAPY (ADEPT) [J].