Development of a Rapid ATP Bioluminescence Assay for Biocidal Susceptibility Testing of Rapidly Growing Mycobacteria

被引：19

作者：

Kapoor, Renuka ^{[1
]}

Yadav, Jagjit S. ^{[1
]}

机构：

[1] Univ Cincinnati, Coll Med, Dept Environm Hlth, Microbial Pathogenesis Lab, Cincinnati, OH 45267 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2010年 / 48卷 / 10期

关键词：

2-PERCENT ALKALINE GLUTARALDEHYDE; METALWORKING FLUIDS; IMMUNOGENUM; IDENTIFICATION; EXTRACTION; ABSCESSUS; CHELONAE; MULTIPLE;

D O I：

10.1128/JCM.01482-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An ATP-based biocide susceptibility assay for mycobacteria was developed by optimizing the cell lysis and assay conditions. Compared to the conventional agar plating method, the assay was rapid (1.5 h) and showed high sensitivity and specificity as determined by receiver operating characteristic (ROC) analysis. The test species, Mycobacterium immunogenum, M. chelonae, and M. abscessus, showed various susceptibilities to the glutaraldehyde- and isothiazolone-based test biocides.

引用

页码：3725 / 3728

页数：4

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