Laminin-5 promotes neurite outgrowth from central and peripheral chick embryonic neurons

被引：23

作者：

Culley, B

Murphy, J

Babaie, J

Nguyen, D

Pagel, A

Rousselle, P

Clegg, DO ^{[2
]}

机构：

[1] Inst Biol & Chim Prot, Lyon, France

[2] Univ Calif Santa Barbara, Neurosci Res Inst, Santa Barbara, CA 93106 USA

[3] Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA

来源：

NEUROSCIENCE LETTERS | 2001年 / 301卷 / 02期

关键词：

laminin-5; neurite outgrowth assay; calcein AM; extracellular matrix;

D O I：

10.1016/S0304-3940(01)01615-9

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Laminin-5 (Ln-5) is an essential component of epithelial basal laminae that is also expressed in the developing nervous system. Here we use a convenient, simple and reproducible in vitro fluorescent assay to assess the neurite outgrowth promoting activity of purified Ln-5. Embryonic chick neurons from dorsal root ganglia, ciliary ganglia, and (to a lesser extent) retina extended neurites on Ln-5, but the neurite outgrowth promoting activity was not as great as that of Ln-l or Ln-2. Neurons from diencephalon, telencephalon, and spinal cord did not respond to Ln-5. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：83 / 86

页数：4

共 20 条

[1] DEVELOPMENTAL EXPRESSION OF NICEIN ADHESION PROTEIN (LAMININ-5) SUBUNITS SUGGESTS MULTIPLE MORPHOGENIC ROLES [J].