Development and Evaluation of a Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Enterovirus 71

被引:30
作者
Jiang, Tao [1 ]
Liu, Juan [2 ]
Deng, Yong-Qiang [1 ]
Xu, Li-Juan [1 ]
Li, Xiao-Feng [1 ]
Han, Jian-Feng [1 ]
Cao, Rui-Yuan [1 ]
Qin, E-De [1 ]
Qin, Cheng-Feng [1 ]
机构
[1] Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China
[2] PLA AF, Gen Hosp, Dept Transfus, Beijing 100142, Peoples R China
关键词
MOUTH-DISEASE; PANDEMIC INFLUENZA; RT-LAMP; VIRUS; FOOT; HAND; INFECTION; OUTBREAK; DIFFERENTIATION; EPIDEMIOLOGY;
D O I
10.1128/JCM.02045-10
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Human enterovirus 71 (EV71) is the major etiological agent of hand, foot, and mouth disease (HFMD), which is a common infectious disease in young children and infants. EV71 can cause various clinical manifestations and has been associated with severe neurological complications; it has resulted in fatalities during recent outbreaks in Asian-Pacific regions since 1997. The early and rapid detection is critical for prevention and control of EV71 infection, since no vaccine or antiviral drugs are currently available. In this study, a simple and sensitive reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay was developed for rapid detection of EV71. The detection limit of the RT-LAMP assay was approximately 0.01 PFU per reaction mixture, and no cross-reactive amplification with other enteroviruses was observed. The assay was evaluated further with 40 clinical specimens and exhibited 92.9% sensitivity and 100% specificity. This RT-LAMP assay may become a useful alternative in clinical diagnosis of EV71, especially in resource-limited hospitals or rural clinics of China and other countries in the Asian-Pacific region.
引用
收藏
页码:870 / 874
页数:5
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