Aptameric enzyme subunit for homogeneous DNA sensing

被引：16

作者：

Ikebukuro, Kazunori ^{[1
]}

Yoshida, Wataru ^{[1
]}

Sode, Koji ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol & Life Sci, Koganei, Tokyo 1848588, Japan

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 02期

关键词：

aptameric enzyme subunit; enzyme-inhibiting aptamer; homogeneous DNA sensing;

D O I：

10.1007/s10529-007-9526-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have developed an aptameric enzyme subunit (AES) which can detect the DNA in a homogeneous solution. The AES is an artificial enzyme subunit composed of an enzyme-inhibiting aptamer bearing a target-molecule binding site. We connected a probe DNA to a thrombin-inhibiting aptamer at its 5' or 3' end. The inhibitory activity of the thrombin-inhibiting aptamer bearing the probe DNA decreased compared to that of the original aptamer; however, it recovered upon hybridization with the target DNA. Using this AES, we were able to detect target DNAs by measuring the thrombin activity in a homogeneous solution.

引用

页码：243 / 252

页数：10

共 27 条

[1] SELECTION OF SINGLE-STRANDED-DNA MOLECULES THAT BIND AND INHIBIT HUMAN THROMBIN [J].

BOCK, LC ;

GRIFFIN, LC ;

LATHAM, JA ;

VERMAAS, EH ;

TOOLE, JJ .

NATURE, 1992, 355 (6360) :564-566

[2] Biophysical and biological properties of quadruplex oligodeoxyribonucleotides [J].

Dapic, V ;

Abdomerovic, V ;

Marrington, R ;

Peberdy, J ;

Rodger, A ;

Trent, JO ;

Bates, PJ .

NUCLEIC ACIDS RESEARCH, 2003, 31 (08) :2097-2107

[3] INVITRO SELECTION OF RNA MOLECULES THAT BIND SPECIFIC LIGANDS [J].

ELLINGTON, AD ;

SZOSTAK, JW .

NATURE, 1990, 346 (6287) :818-822

[4] Detection of influenza A viruses from different species by PCR amplification of conserved sequences in the matrix gene [J].

Fouchier, RAM ;

Bestebroer, TM ;

Herfst, S ;

Van der Kemp, L ;

Rimmelzwaan, GF ;

Osterhaus, ADME .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (11) :4096-4101

[5]

GALAN JE, 1990, INFECT IMMUN, V58, P1879

[6]

Ho HA, 2002, ANGEW CHEM, V114, P1618

[7] A novel method of screening thrombin-inhibiting DNA aptamers using an evolution-mimicking algorithm [J].

Ikebukuro, K ;

Okumura, Y ;

Sumikura, K ;

Karube, I .

NUCLEIC ACIDS RESEARCH, 2005, 33 (12) :1-7

[8] Rapid and specific detection of PCR products using light-up probes [J].

Isacsson, J ;

Cao, H ;

Ohlsson, L ;

Nordgren, S ;

Svanvik, N ;

Westman, G ;

Kubista, M ;

Sjöback, R ;

Sehlstedt, U .

MOLECULAR AND CELLULAR PROBES, 2000, 14 (05) :321-328

[9] A double chain reversal loop and two diagonal loops define the architecture of a unimolecular DNA quadruplex containing a pair of stacked G(syn)•G(syn)•G(anti)•G(anti) tetrads flanked by a G•(T-T) triad and a T•T•T triple [J].

Kuryavyi, V ;

Majumdar, A ;

Shallop, A ;

Chernichenko, N ;

Skripkin, E ;

Jones, R ;

Patel, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 2001, 310 (01) :181-194

[10] A new class of homogeneous nucleic acid probes based on specific displacement hybridization [J].

Li, QQ ;

Luan, GY ;

Guo, QP ;

Liang, JX .

NUCLEIC ACIDS RESEARCH, 2002, 30 (02) :5

← 1 2 3 →