Expression and characterization of PP7, a novel plant protein Ser/Thr phosphatase distantly related to RdgC/PPEF and PP5

被引：24

作者：

Kutuzov, MA ^{[1
]}

Evans, DE ^{[1
]}

Andreeva, AV ^{[1
]}

机构：

[1] Oxford Brookes Univ, Res Sch Biol & Mol Sci, Oxford OX3 0BP, England

来源：

FEBS LETTERS | 1998年 / 440卷 / 1-2期

关键词：

protein Ser/Thr phosphatase; PP7; okadaic acid; calyculin A; fumonisin B-1; Arabidopsis thaliana;

D O I：

10.1016/S0014-5793(98)01428-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have recently identified an Arabidopsis thaliana cDNA encoding a putative protein Ser/Thr phosphatase PP7, not closely related to any protein phosphatases in animals or fungi, Here, we describe the characterization of PP7 expressed in a bacterial system, The recombinant protein was inactive unless the longest insert in its catalytic domain was cleaved, suggesting that this insert is an autoinhibitory region. PP7 was resistant to okadaic acid, calyculin and fumonisin B-1, and was stimulated by Mn2+ or Fe2+, while Ni2+ and Zn2+ were inhibitory. Polylysine stimulated PP7 activity towards p-nitrophenylphosphate but inhibited activity towards the most efficient protein substrate, myelin basic protein. A tentative model of the control of PP7 activity is proposed. (C) 1998 Federation of European Biochemical Societies.

引用

页码：147 / 152

页数：6

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