Separation and Purification of Gardecin from Gardenia jasminoides Ellis by High-Speed Countercurrent Chromatography

被引：6

作者：

Chen, Yang ^{[1
]}

Deng, Liang ^{[2
,3
]}

Wang, Wenjun ^{[1
]}

Xia, Qiang ^{[1
]}

Zhou, Xumei ^{[4
]}

Wang, Yarong ^{[1
]}

Hou, Huijing ^{[1
]}

Cai, Shining ^{[1
]}

Cui, Guozhen ^{[1
]}

Chen, Ping ^{[5
]}

Lu, Tao ^{[5
]}

Zhang, Hao ^{[5
]}

机构：

[1] Zunyi Med Coll, Dept Pharmaceut Sci, Zhuhai 51904, Guangdong, Peoples R China

[2] Kunming Med Univ, Sch Pharmaceut Sci, Kunming 650500, Yunnan, Peoples R China

[3] Kunming Med Univ, Yunnan Key Lab Pharmacol Nat Prod, Kunming 650500, Yunnan, Peoples R China

[4] Zunyi Med Univ, Dept Pharmaceut Anal, Zunyi, Guizhou, Peoples R China

[5] Sichuan Univ, West China Sch Pharm, Chengdu 610064, Sichuan, Peoples R China

来源：

SEPARATION SCIENCE AND TECHNOLOGY | 2015年 / 50卷 / 12期

关键词：

gardecin; separation and purification; crocetin derivative (crocin); high-speed counter-current chromatography (HSCCC); antioxidant activity; Gardenia jasminoides Ellis; PREPARATIVE ISOLATION; ANTIOXIDANT ACTIVITY; CROCIN;

D O I：

10.1080/01496395.2015.1013636

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

In the present study, a high-speed counter-current chromatography (HSCCC) was investigated for separation and purification of gardecin on a preparative scale. Hexane-ethyl acetate-methanol-water (0.3:1:0.3:1, v/v) was selected as the optimum solvent system to purify gardecin from a fraction obtained from HPD100 column chromatography fractionation of gardenia. After HSCCC isolation, 20.1 mg of gardecin with purity of 97.4% was obtained from 322 g of dry gardenia fruits. Chemical structure identification of this pigment was carried out by MS, H-1 NMR, and C-13 NMR. Additionally, antioxidant activity of gardecin in comparison with crocin-1 was investigated. The present results demonstrated that gardecin could be efficiently obtained using HSCCC from this herb and this compound features strong antioxidant activity.

引用

页码：1899 / 1905

页数：7

共 12 条

[1]

Antioxidant potential of crocins and ethanol extracts of Gardenia jasminoides ELLIS and Crocus sativus L.:: A relationship investigation between antioxidant activity and crocin contents [J].

Chen, Yang ;

Zhang, Hao ;

Tian, Xi ;

Zhao, Can ;

Cai, Le ;

Liu, Ying ;

Jia, Lin ;

Yin, Hong-Xiang ;

Chen, Chu .

FOOD CHEMISTRY, 2008, 109 (03) :484-492

[2]

Spectroscopic, stability and radical-scavenging properties of a novel pigment from gardenia [J].

Chen, Yang ;

Cai, Le ;

Zhao, Can ;

Xu, Hui-Chuan ;

Cao, Cheng-yong ;

Liu, Ying ;

Jia, Lin ;

Yin, Hong-Xiang ;

Chen, Chu ;

Zhang, Hao .

FOOD CHEMISTRY, 2008, 109 (02) :269-277

[3]

Crocin and geniposide profiles and radical scavenging activity of gardenia fruits (Gardenia jasminoides Ellis) from different cultivars and at the various stages of maturation [J].

Chen, Yang ;

Zhang, Hao ;

Li, Yi-Xin ;

Cai, Le ;

Huang, Juan ;

Zhao, Can ;

Jia, Lin ;

Buchanan, Ryan ;

Yang, Ting ;

Jiang, Li-Juan .

FITOTERAPIA, 2010, 81 (04) :269-273

[4]

COIL PLANET CENTRIFUGE [J].

ITO, Y ;

WEINSTEIN, M ;

AOKI, I ;

HARADA, R ;

KIMURA, E ;

NUNOGAKI, K .

NATURE, 1966, 212 (5066) :985-+

[5]

Isolation of chlorophylls a and b from spinach by counter-current chromatography [J].

Jubert, Carole ;

Bailey, George .

JOURNAL OF CHROMATOGRAPHY A, 2007, 1140 (1-2) :95-100

[6]

Mitra SK, 1999, PHYTOTHER RES, V13, P300, DOI 10.1002/(SICI)1099-1573(199906)13:4<300::AID-PTR440>3.0.CO

[7]

2-A

[8]

Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E [J].

Prieto, P ;

Pineda, M ;

Aguilar, M .

ANALYTICAL BIOCHEMISTRY, 1999, 269 (02) :337-341

[9]

Preparative isolation and purification of flavone compounds from Sophora japonica L. by highspeed counter-current chromatography combined with macroporous resin column separation [J].

Sun, Ailing ;

Sun, Qinghua ;

Liu, Renmin .

JOURNAL OF SEPARATION SCIENCE, 2007, 30 (07) :1013-1018

[10]

Extraction of isoflavones from stem of Pueraria lobata (Willd.) Ohwi using n-butanol/water two-phase solvent system and separation of daidzein [J].

Xu, Hua-Neng ;

He, Chao-Hong .

SEPARATION AND PURIFICATION TECHNOLOGY, 2007, 56 (01) :85-89

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